Concordant evolution of a symbiont with its host insect species: Molecular phylogeny of genus Glossina and its bacteriome-associated endosymbiont, Wigglesworthia glossinidia
Concordant evolution of a symbiont with its host insect species: Molecular phylogeny of genus Glossina and its bacteriome-associated endosymbiont, Wigglesworthia glossinidia
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DOI：
10.1007/pl00006444
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发表时间：
1999-01-01
影响因子：
3.9
通讯作者：
Aksoy, S
中科院分区：
文献类型：
--
作者：
Chen, XA;Li, S;Aksoy, S
许多限制饮食的节肢动物依靠共生关系来获得充分的营养和繁殖力。采采蝇（双翅目：舌蝇科）除了传播非洲锥虫外，还携带三种共生生物。其中两种微生物存在于不同的肠道细胞中，而第三种微生物存在于生殖组织中，属于沃尔巴克氏菌属。初级共生体（Wigglesworthia gloss属）生活在分化的上皮细胞（细菌细胞）中，这些细胞在前肠形成一个器官（细菌组），而次级共生体存在于中肠细胞中。本文通过对Glossina 3个亚属（=fusca group）、Nemorhina （=palpalis group）和Glossina (=morsitans group) 8个种的16S rDNA序列分析，对Wigglesworthia的系统发育进行了初步表征。独立地分析了这些物种的核糖体DNA内部转录间隔-2 （ITS-2）区域。Wigglesworthia的分析表明，它们在变形菌门γ亚群中形成了一个独特的谱系，并与它们的宿主昆虫物种表现出一致性。由parsimony生成的树证实了Glossina的单系分类位置，其中fusca类群形成最深的分支，其次是morsitans和palpalis类群。基于ITS-2和共生体16S rDNA序列分析的结果表明，Glossina austeni应该被放置在一个单独的第四亚属Machadomyia中，该亚属与morsitans类群形成姐妹群关系。
Many arthropods with restricted diets rely on symbiotic associations for full nutrition and fecundity. Tsetse flies (Diptera: Glossinidae) harbor three symbiotic organisms in addition to the parasitic African trypanosomes they transmit. Two of these microorganisms reside in different gut cells, while the third organism is harbored in reproductive tissues and belongs to the genus Wolbachia. The primary symbiont (genus Wigglesworthia glossinidia) lives in differentiated epithelial cells (bacteriocytes) which form an organ (bacteriome) in the anterior gut, while the secondary (S) symbionts are present in midgut cells. Here we have characterized the phylogeny of Wigglesworthia based on their 16S rDNA sequence analysis from eight species representing the three subgenera of Glossina: Austenina (=fusca group), Nemorhina (=palpalis group), and Glossina (=morsitans group). Independently, the ribosomal DNA internal transcribed spacer-2 (ITS-2) regions from these species were analyzed. The analysis of Wigglesworthia indicated that they form a distinct lineage in the gamma subdivision of Proteobacteria and display concordance with their host insect species. The trees generated by parsimony confirmed the monophyletic taxonomic placement of Glossina, where fusca group species formed the deepest branch followed by morsitans and palpalis groups, respectively. The placement of the species Glossina austeni by both the traditional morphological and biochemical criteria has been controversial, Results presented here, based on both the ITS-2 and the symbiont 16S rDNA sequence analysis, suggest that Glossina austeni should be placed into a separate fourth subgenus, Machadomyia, which forms a sister-group relationship with the morsitans group species.