A GENETIC-LOCUS OF ENTEROCYTE EFFACEMENT CONSERVED AMONG DIVERSE ENTEROBACTERIAL PATHOGENS
A GENETIC-LOCUS OF ENTEROCYTE EFFACEMENT CONSERVED AMONG DIVERSE ENTEROBACTERIAL PATHOGENS
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DOI：
10.1073/pnas.92.5.1664
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发表时间：
1995-02-28
影响因子：
11.1
通讯作者：
KAPER, JB
中科院分区：
文献类型：
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作者：
MCDANIEL, TK;JARVIS, KG;KAPER, JB
肠致病性大肠埃希菌(EPEC)和肠出血性大肠杆菌O157：H7是肠道病原菌，对上皮细胞的微绒毛和顶下细胞骨架有严重的损伤作用。在这里，我们报告在EPEC中发现一个35kBP的基因座，其中包含几个与这些病变的形成有关的区域。整个DNA探针与O157：H7和其他三个属的病原体杂交，这些病原体引起类似的病变，但不与同一物种的无毒成员杂交。尿路致病性大肠杆菌的EPEC基因座和不同的毒力基因座插入到大肠杆菌染色体上的相同位置，并在插入点附近拥有高度相似的序列。
Enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli O157:H7 are intestinal pathogens that profoundly damage the microvilli and subapical cytoskeleton of epithelial cells. Here we report finding in EPEC a 35-kbp locus containing several regions implicated in formation of these lesions. DNA probes throughout this locus hybridize to E. coli O157:H7 and other pathogens of three genera that cause similar lesions but do not hybridize to avirulent members of the same species. The EPEC locus and a different virulence locus of uropathogenic E. coli insert into the E. coli chromosome at the identical site and share highly similar sequences near the point of insertion.