Precise Control of Movement Kinematics by Optogenetic Inhibition of Purkinje Cell Activity
Precise Control of Movement Kinematics by Optogenetic Inhibition of Purkinje Cell Activity
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DOI：
10.1523/jneurosci.4547-13.2014
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发表时间：
2014-02-05
影响因子：
5.3
通讯作者：
Medina, Javier F.
中科院分区：
文献类型：
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作者：
Heiney, Shane A.;Kim, Jinsook;Medina, Javier F.
小脑皮质的浦肯野细胞（PC）是精确控制运动所必需的，但这些抑制性神经元的活动如何调节运动输出的机制解释仍然缺乏。我们在清醒的小鼠中使用光遗传学方法，首次表明短暂抑制PC群体中的自发活动足以引起离散运动，这些运动可以在大小，速度和时间上进行系统调节，这取决于PC放电被抑制的程度和时间。我们进一步证明，这种精细的运动学控制介导的分级解除抑制的靶神经元在小脑深核。我们的研究结果证明了小脑功能的长期模型，并首次证明抑制信号的抑制可以作为精确控制行为的强大机制。
Purkinje cells (PCs) of the cerebellar cortex are necessary for controlling movement with precision, but a mechanistic explanation of how the activity of these inhibitory neurons regulates motor output is still lacking. We used an optogenetic approach in awake mice to show for the first time that transiently suppressing spontaneous activity in a population of PCs is sufficient to cause discrete movements that can be systematically modulated in size, speed, and timing depending on how much and how long PC firing is suppressed. We further demonstrate that this fine control of movement kinematics is mediated by a graded disinhibition of target neurons in the deep cerebellar nuclei. Our results prove a long-standing model of cerebellar function and provide the first demonstration that suppression of inhibitory signals can act as a powerful mechanism for the precise control of behavior.