Ectomycorrhizal fungal community of naturally regenerated Pinus thunbergii seedlings in a coastal pine forest
Ectomycorrhizal fungal community of naturally regenerated Pinus thunbergii seedlings in a coastal pine forest
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DOI：
10.1007/s10310-009-0140-x
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发表时间：
2009-12-01
影响因子：
1.5
通讯作者：
Ito, Shin-ichiro
中科院分区：
文献类型：
--
作者：
Matsuda, Yosuke;Noguchi, Yuuta;Ito, Shin-ichiro
为了增加外生菌根（ECM）的知识，在日本沿海森林的黑松幼苗的根的群落结构，我们采样自然再生的本年度和1至5岁的幼苗。根据形态学和遗传学分析，将当年生苗的667个根尖和老苗的1,927个根尖分为13个类型。土生空球菌（Cenococcum geophilum）是棒菌科（Clavulinaceae）、红菇科（Russulaceae）、革菌科（Thelephoraceae）或毛壳菌属（Trichophaea）的成员，被指示为形成雷氏外生菌根的真菌。其中，C. geophilum和Clavulinaceae sp.1为最大或第二大优势种。基于样本数量的物种积累曲线几乎达到了一个平台，观察到的物种丰富度等于11种，Jackknife 2和Chao 2丰富度估计分别表示14和12种。Simpson's 1/D为3.89，Shannon-Wiener's H'为1.71，分类多样性较低。外生菌根形成率、外生菌根类型的出现频率和数量在当年生和老苗之间没有显著差异。这些结果表明，较少的多样性真菌参与外生菌根形成的沿海松树幼苗相比，在研究区的可比内陆森林。
To add to knowledge of ectomycorrhizal (ECM) community structure on the roots of Pinus thunbergii seedlings in a Japanese coastal forest, we sampled naturally regenerated current-year and 1- to 5-year-old seedlings. We classified the 667 root tips on current-year seedlings and the 1,927 root tips on older seedlings into 13 phylotypes based on morphological and genetic analyses. Cenococcum geophilum, members of the families Clavulinaceae, Russulaceae, or Thelephoraceae or the genus Trichophaea, were indicated to be fungi forming P. thunbergii ectomycorrhizas. Among them, C. geophilum and Clavulinaceae sp. 1 were the most or second most dominant species. A species accumulation curve based on the number of samples nearly reached a plateau, with observed species richness equal to 11 species and the Jackknife2 and Chao2 richness estimators indicating 14 and 12 species, respectively. In addition, Simpson's 1/D was 3.89 and Shannon-Wiener's H' was 1.71, indicating a relatively low taxonomic diversity. There was no significant difference in the ectomycorrhizal formation rate, or the occurrence frequency and the number of ECM phylotypes between current-year and older seedlings. These results indicated that less diverse fungi were involved in ectomycorrhizal formation on coastal pine seedlings compared with those in comparable inland forests in the study area.