Ultrasonic deposition of cells on a surface
Ultrasonic deposition of cells on a surface
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DOI：
10.1016/j.bios.2003.10.003
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发表时间：
2004-04-15
影响因子：
12.6
通讯作者：
McDonnell, MB
中科院分区：
文献类型：
--
作者：
Hawkes, JJ;Long, MJ;McDonnell, MB
水中的细菌被超声驻波驱赶到玻璃表面。在抗体包被的表面上，枯草芽孢杆菌黑色尼日尔（BG）孢子的捕获（6.6 × 10（6）ml（-1））比在没有超声的情况下的效率增加超过200倍。在微流体（非湍流）系统中，通过在表面处操作的传感器检测颗粒是扩散受限的。这导致非常低的检测能力，特别是对于直径大于1 μ m的颗粒。这里使用超声波将细菌孢子驱动到墙壁上，并克服了这一限制。结果证实：（I）当玻璃厚度接近超声波长的一半时，可以在接近水-玻璃界面处形成压力节点（2）所使用的抗体能够在所有超声驻波存在下捕获孢子。(C)2003 Elsevier B. V.保留所有权利。
Bacteria in water have been driven to a glass surface by all ultrasonic standing wave. On an antibody coated surface Capture of Bacillus subtilis var niger (BG) spores (6.6 x 10(6) ml(-1)) was increased more than 200-fold over above the efficiency in the absence of ultrasound. In microfluidic (non-turbulent) systems detection of particles by sensors operating at a surface is diffusion limited. This results in very low detection abilities particularly for particles with diameters greater than 1 mum. Ultrasound is used here to drive bacterial spores to a wall and overcome this limitation. The results confirm: (I) pressure nodes can be formed close to the water-glass interface when the glass thickness is near half the ultrasonic wavelength (2) the antibody used was able to capture spores in the presence Of all ultrasonic standing wave. (C) 2003 Elsevier B.V. All rights reserved.