Increased freezing tolerance in an ABA-hypersensitive mutant of common wheat
Increased freezing tolerance in an ABA-hypersensitive mutant of common wheat
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DOI：
10.1016/j.jplph.2006.11.004
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Nakamura, Chiharu
中科院分区：
文献类型：
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作者：
Kobayashi, Fuminori;Takumi, Shigeo;Nakamura, Chiharu
为了研究脱落酸（ABA）在小麦冷驯化以及抗寒/抗冻性中的作用，我们分析了一个普通小麦（Triticum aestivum）对ABA超敏感的突变体，命名为ABA27。通过涉及萌发和幼苗生长的生物测定以及与亲本品种“秩父小麦”（Chihoku）相比ABA响应基因的表达分析，证实了ABA27对ABA的超敏感性。ABA27在未经冷驯化的幼苗中表现出显著提高的抗冻性。在正常和低温（LT）条件下，ABA处理的ABA27幼苗比秩父积累了更多的ABA响应基因Cor/Lea及其假定的转录因子（TF）基因的转录本。在正常温度下，非ABA调节的Cor/Lea转录本在ABA27中也有更高的积累。这些结果表明，ABA27中提高的ABA敏感性通过增加ABA调节的低温信号通路的表达有助于提高抗冻性。基于这些结果以及在一个对ABA不太敏感的突变体中获得的先前结果，表明ABA反应的正向和负向调节都参与了小麦抗冻性的基本机制。（c）2006爱思唯尔有限公司。保留所有权利。
To study role of abscisic acid (ABA) in cold acclimation and cold/freezing tolerance in wheat, we analyzed an ABA-hypersensitive mutant of Triticum aestivum, named ABA27. ABA-hypersensitivity in ABA27 was confirmed by bioassays involving germination and seedling growth and expression analysis of ABA-responsive genes in comparison with the parental cultivar 'Chihoku-komugi' (Chihoku). ABA27 showed significantly increased freezing tolerance in seedlings without cold acclimation. ABA-treated seedlings of ABA27 accumulated more transcripts of ABA-responsive genes Cor/Lea and their putative transcription factor (TF) genes than Chihoku under both normal and low-temperature (LT) conditions. Non-ABA-regulated Cor/Lea transcripts showed higher accumulation in ABA27 also under normal temperature. These results suggest that the elevated ABA sensitivity in ABA27 contributes to the improved freezing tolerance through increased expression of the ABA-regulated LT signal pathway. Based on these and previous results obtained in an ABA-less-sensitive mutant, it is suggested that both positive and negative regulation of ABA response is involved in the basic mechanism of freezing tolerance in wheat. (c) 2006 Elsevier GmbH. All rights reserved.