Differential regulation of fluorescent alkaloid metabolism between idioblast and lacticifer cells during leaf development in Catharanthus roseus seedlings
Differential regulation of fluorescent alkaloid metabolism between idioblast and lacticifer cells during leaf development in Catharanthus roseus seedlings
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DOI：
10.1007/s10265-022-01380-1
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发表时间：
2022-03
影响因子：
2.8
通讯作者：
Mai Uzaki;Kotaro Yamamoto;A. Murakami;Yushiro Fuji;M. Ohnishi;K. Ishizaki;H. Fukaki;M. Hirai;T. Mimura
中科院分区：
文献类型：
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作者：
Mai Uzaki;Kotaro Yamamoto;A. Murakami;Yushiro Fuji;M. Ohnishi;K. Ishizaki;H. Fukaki;M. Hirai;T. Mimura
具有生物活性的特化（次生）代谢物是植物发育或适应周围环境所不可缺少的。在许多植物中，这些特化代谢物在特异性分化的细胞中积累。玫瑰花是一种著名的药用植物，以生产多种单萜类吲哚生物碱而闻名。玫瑰有两种类型的特异性分化细胞，即所谓的异母细胞和乳汁细胞。在本研究中，我们比较了每个细胞在幼苗生长过程中的变化，发现这些细胞中积累的荧光代谢物受到不同的调节。荧光化合物分析表明，在这些细胞中观察到的荧光是由化合物蛇纹石发出的。此外，我们发现叶片的蛇纹石含量随着叶片的生长而增加。本研究结果提示异母细胞和乳汁细胞在MIA生物合成及其调控中具有不同的生物学作用。
Bioactive specialized (secondary) metabolites are indispensable for plant development or adjustment to their surrounding environment. In many plants, these specialized metabolites are accumulated in specifically differentiated cells.Catharanthus roseusis a well-known medicinal plant known for producing many kinds of monoterpenoid indole alkaloids (MIAs).C. roseushas two types of specifically differentiated cells accumulating MIAs, so-called idioblast cells and laticifer cells. In this study, we compared each of the cells as they changed during seedling growth, and found that the fluorescent metabolites accumulated in these cells were differentially regulated. Analysis of fluorescent compounds revealed that the fluorescence observed in these cells was emitted from the compound serpentine. Further, we found that the serpentine content of leaves increased as leaves grew. Our findings suggest that idioblast cells and laticifer cells have different biological roles in MIA biosynthesis and its regulation.