Design of an optical switch for studying conformational dynamics in individual molecules of GroEL
Design of an optical switch for studying conformational dynamics in individual molecules of GroEL
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DOI：
10.1021/bc800118j
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发表时间：
2008-07-01
影响因子：
4.7
通讯作者：
Haran, Gilad
中科院分区：
文献类型：
--
作者：
Frank, Gabriel A.;Kipnis, Yakov;Haran, Gilad
我们描述了一个光开关的伴侣GroEL是打开和关闭其ATP和cochaperonin GroES驱动的构象变化的设计。基于荧光团和猝灭剂的开关被设计成分子伴侣的单环变体，并且响应于蛋白质在其变构状态之间的转变而显示其荧光强度的显著调制。因此，它形成了一个敏感的探针，在散装和单分子中，这台机器的变构转换的动力学。
We describe the design of an optical switch in the chaperonin GroEL that is opened and closed by its ATP- and cochaperonin GroES-driven conformational changes. The switch, based on a fluorophore and a quencher, is engineered into the single-ring variant of the chaperone, and shows dramatic modulation of its fluorescent intensity in response to the transition of the protein between its allosteric states. It, therefore, forms a sensitive probe for the dynamics of the allosteric transitions of this machine, both in the bulk and in single molecules.