Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells
Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells
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DOI：
10.1016/j.lfs.2009.02.006
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发表时间：
2009-04-24
期刊：
影响因子：
6.1
通讯作者：
Kashiwakura, Ikuo
中科院分区：
文献类型：
--
作者：
Hayashi, Naoki;Takahashi, Kenji;Kashiwakura, Ikuo
目的：本文评估了从胎盘/脐带血制备的人间充质干细胞样基质通过X-照射的造血干细胞进行造血再生的潜力。将胎盘/脐带血来源的间充质干细胞样基质细胞应用于X射线照射的人CD 34（+）细胞的再生性离体扩增，所述细胞在补充有白细胞介素-β 2受体拮抗剂组合的无血清液体培养物中。3加干细胞因子加血小板生成素。主要发现：在未照射和X-照射的细胞与基质细胞的共培养中产生的细胞和谱系定型髓系造血祖细胞的总数显著高于无基质培养中的细胞和谱系定型髓系造血祖细胞。此外，CD 34（+）细胞和CD 34（+）/CD 38（-）细胞、未成熟造血干/祖细胞的数量也较无基质培养增加。基质细胞产生各种类型的细胞因子，虽然没有照射和X射线照射的细胞与基质细胞的共培养物之间的差异不大。此外，当X射线照射的细胞与基质细胞接触16小时，细胞因子刺激前，观察到类似程度的造血，从而表明细胞与细胞的相互作用的关键作用。意义：目前的结果表明，从照射造血干/祖细胞造血再生的人间充质干细胞样基质的潜在功效。(C)2009 Elsevier Inc. All rights reserved.
Aims: The potential of human mesenchymal stem cell-like stroma prepared from placental/umbilical cord blood for hematopoietic regeneration by X-irradiated hematopoietic stem cells is herein assessed.Main methods: Placental/ umbilical cord blood-derived mesenchymal stem cell-like stromal cells were applied to a regenerative ex vivo expansion of X-irradiated human CD34(+) cells in a serum-free liquid culture supplemented with a combination of interleukine-3 plus stem cell factor plus thrombopoietin.Key findings: The total number of cells and of lineage-committed myeloid hematopoietic progenitor cells generated in the co-culture of both non-irradiated and X-irradiated cells with stromal cells was significantly higher than those in the stroma-free culture. In addition, the number of CD34(+) cells and CD34(+)/CD38(-) cells, immature hematopoietic stem/progenitor cells also increased more than the stroma-free culture. The stromal cells produced various types of cytokines, although there was little difference between the co-cultures of non-irradiated and X-irradiated cells with stromal cells. Furthermore, when X-irradiated cells came in contact with stromal cells for 16 h before cytokine stimulation, a similar degree of hematopoiesis was observed, thus suggesting the critical role of cell-to-cell interaction.Significance: The present results showed the potential efficacy of human mesenchymal stem cell-like stroma for hematopoietic regeneration from irradiated hematopoietic stem/progenitor cells. (C) 2009 Elsevier Inc. All rights reserved.