PCL-PEG-PCL film promotes cartilage regeneration in vivo
PCL-PEG-PCL film promotes cartilage regeneration in vivo
复制标题
PCL-PEG-PCL膜促进体内软骨再生。
DOI：
10.1111/cpr.12295
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发表时间：
2016-12-01
影响因子：
8.5
通讯作者：
Lin, Yunfeng
中科院分区：
文献类型：
--
作者：
Fu, Na;Liao, Jinfeng;Lin, Yunfeng
目的由于关节软骨的自愈能力差，软骨缺损的治疗长期以来一直是一个挑战。许多方法，从对症治疗到结构性软骨再生，都获得了非常有限的令人满意的结果。软骨组织工程涉及新型支架、细胞来源和生长因子的优化组合，已成为软骨再生和修复的一种有前景的策略。本研究旨在探讨聚(ε-己内酯)-聚(乙二醇)-聚(ε-己内酯)(PCL-PEG-PCL, PCEC) PCEC支架在软骨修复中的作用。材料与方法首先制备PCEC薄膜，并使用SEM和AFM测试其特性。细胞接种后，使用 SEM 和荧光显微镜观察 PCEC 膜上的细胞（rASC - 大鼠脂肪源性干细胞和 mASC - 绿色荧光小鼠脂肪源性干细胞）形态。使用 CCK-8 测定法对 PCEC 膜上的细胞活力进行测试。此外，在大鼠体内制作全软骨缺损，并植入PCEC薄膜，以评估其愈合效果，历时8周。结果发现PCEC薄膜作为生物材料植入物，具有良好的体外细胞粘附、迁移和增殖特性。重要的是，在体内实验中，PCEC膜表现出理想的愈合效果。结论这些结果表明PCEC膜是软骨组织工程的良好支架，可改善细胞增殖和粘附，并可实现软骨缺损的良好修复。
ObjectiveManagement of chondral defects has long been a challenge due to poor self-healing capacity of articular cartilage. Many approaches, ranging from symptomatic treatment to structural cartilage regeneration, have obtained very limited satisfactory results. Cartilage tissue engineering, which involves optimized combination of novel scaffolds, cell sources and growth factors, has emerged as a promising strategy for cartilage regeneration and repair. In this study, the aim was to investigate the role of poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCL-PEG-PCL, PCEC) PCEC scaffold in cartilage repair.Materials and methodsFirst, PCEC film was fabricated, and its characteristics were tested using SEM and AFM. Cell (rASC - rat adipose-derived stem cells, and mASCs - green fluorescent mouse adipose-derived stem cells) morphologies on PCEC film were observed using SEM and fluorescence microscopy, after cell seeding. Tests of cell viability on PCEC film were conducted using the CCK-8 assay. Furthermore, full cartilage defects in rats were created, and PCEC films were implanted, to evaluate their healing effects, over 8weeks.ResultsIt was found that PCEC film, as a biomaterial implant, possessed good in vitro properties for cell adhesion, migration and proliferation. Importantly, in the in vivo experiment, PCEC film exhibited desirable healing outcomes.ConclusionsThese results demonstrated that PCEC film was a good scaffold for cartilage tissue engineering for improving cell proliferation and adhesion and could lead to excellent repair of cartilage defects.