Taxonomic study of aerobic thermophilic bacilli:: descriptions of Geobacillus subterraneus gen. nov., sp nov and Geobacillus uzenensis sp nov from petroleum reservoirs and transfer of Bacillus stearothermophilus Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G-stearothermophilus, G-thermocatenulatus, G-thermoleovorans, G-kaustophilus, G-thermoglucosidasius and G-thermodenitrificans
Taxonomic study of aerobic thermophilic bacilli:: descriptions of Geobacillus subterraneus gen. nov., sp nov and Geobacillus uzenensis sp nov from petroleum reservoirs and transfer of Bacillus stearothermophilus Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G-stearothermophilus, G-thermocatenulatus, G-thermoleovorans, G-kaustophilus, G-thermoglucosidasius and G-thermodenitrificans
复制标题
DOI：
10.1099/00207713-51-2-433
复制
发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Ivanov, MV
中科院分区：
文献类型：
--
作者：
Nazina, TN;Tourova, TP;Ivanov, MV
从俄罗斯、哈萨克斯坦和中国的油田地层沃茨中分离到5株烃氧化菌。这些菌株是中度嗜热、嗜热、能动、孢子形成棒、需氧或兼性厌氧的。DNA的G+C含量为49.7 ~ 52.3mol%。主要的类异戊二烯醌是甲基萘醌-7;细胞脂肪酸谱由显著量的iso-15：0、iso-16：0和iso-17：0脂肪酸组成（占总量的61.7-86.8%）。根据16 S rDNA分析和DNA-DNA杂交结果，地下分离物可分为两大类群，一类群由菌株U-T和X组成，另一类群由菌株K、Sam和34（T）组成。新菌株显示出与Ash等人[Ash，C.，Farrow，J. A. E、沃尔班克斯，S. &柯林斯，M. D.（1991年）。Left Appl Microbiol 13，202-206]和16 S rRNA的一组相应的特征位置。对新分离株和已建立的嗜热杆菌种的16 S rDNA序列和脂肪酸组成的比较分析表明，地下菌株代表了一个新属中的两个新种，其名称为Geobacillussubjumeusgen，nov.，新种，和Geobacillus uzenensis sp，nov.。还建议将嗜热脂肪芽孢杆菌、嗜热噬热芽孢杆菌、热小链芽孢杆菌、嗜碱芽孢杆菌、热葡萄糖酸芽孢杆菌和热脱氮芽孢杆菌转入该新属，其中嗜热脂肪地芽孢杆菌（原嗜热脂肪芽孢杆菌）为模式种。
Five hydrocarbon-oxidizing strains were isolated from formation waters of oilfields in Russia, Karakhstan and China. These strains were moderately thermophilic, neutrophilic, motile, spore-forming rods, aerobic or facultatively anaerobic. The G+C content of their DNA ranged from 49.7 to 52.3 mol%. The major isoprenoid quinone was menaquinone-7; cellular fatty acid profiles consisted of significant amounts of iso-15:0, iso-16:0 and iso-17:0 fatty acids (61.7-86.8% of the total). Based on data from 16S rDNA analysis and DNA-DNA hybridization, the subsurface isolates could be divided into two groups, one of which consisted of strains U-T and X and the other of which consisted of strains K, Sam and 34(T). The new strains exhibited a close phylogenetic relationship to thermophilic bacilli of 'Group 5' of Ash et al. [Ash, C., Farrow, J. A. E., Wallbanks, S. & Collins, M. D. (1991). Left Appl Microbiol 13, 202-206] and a set of corresponding signature positions of 16S rRNA. Comparative analysis of the 16S rDNA sequences and fatty acid compositions of the novel isolates and established species of thermophilic bacilli indicated that the subsurface strains represent two new species within a new genus, for which the names Geobacillus subterraneus gen, nov., sp. nov., and Geobacillus uzenensis sp, nov. are proposed. It is also proposed that Bacillus stearothermophilus, Bacillus thermoleovorans, Bacillus thermocatenulatus, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans be transferred to this new genus, with Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) as the type species.