OSTEOCLASTIC RESORPTION OF CALCIUM PHOSPHATES IS POTENTIATED IN POSTOSTEOGENIC CULTURE CONDITIONS
OSTEOCLASTIC RESORPTION OF CALCIUM PHOSPHATES IS POTENTIATED IN POSTOSTEOGENIC CULTURE CONDITIONS
复制标题
DOI：
10.1002/jbm.820280114
复制
发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
VANBLITTERSWIJK, CA
中科院分区：
文献类型：
--
作者：
DEBRUIJN, JD;BOVELL, YP;VANBLITTERSWIJK, CA
通过使用两种培养系统研究了致密烧结的羟基磷灰石（HA 1250）、磷酸三钙（TCP）和600 ℃或900 ℃煅烧的羟基磷灰石（分别为HA 600和HA 900）的细胞介导的再吸收。第一种是骨细胞培养，第二种是两阶段培养，第一阶段是在基质上形成骨样组织，第二阶段是破骨细胞对骨样组织的吸收。除了对HA 600和HA 900具有有限的吞噬活性外，两种材料在骨细胞培养物中均未显示吸收或表面改变。在两阶段培养中，矿化的细胞外基质的生产只观察到HA 1250和TCP，其随后的破骨细胞样细胞的吸收是显而易见的。小的和偶尔较大的抗酒石酸酸性磷酸酶阳性细胞产生20-150 μ m直径的再吸收坑在两个矿化的细胞外基质上HA 1250和TCP和HA 600和HA 900的表面。TCP上的矿化细胞外基质的再吸收也导致了底层陶瓷表面的降解，主要是从晶界开始，而HA 1250的表面保持不变。这项研究的结果清楚地表明，骨钙素吸收的磷酸钙是加强在成骨后培养条件。假设骨基质成分在细胞介导的吸收中可能发挥作用，而吸收的发生似乎主要受材料特性（如粒度和晶体结构）的综合影响。(C)1994年，John Wiley and Sons，Inc.
Cell-mediated resorption of densely sintered hydroxyapatite (HA1250), tricalcium phosphate (TCP), and 600 degrees or 900 degrees C calcined hydroxyapatite (HA600 and HA900, respectively), was investigated by using two culture systems. The first was an osteoclastic cell culture, and the second was a two-stage culture that was composed of a bonelike tissue formation on the substrata in the first stage and its subsequent resorption by osteoclasts in the second stage. Neither of the materials showed resorption or surface alterations in the osteoclastic cell culture, except for some Limited phagocytotic activity on HA600 and HA900. In the two-stage culture, production of mineralized extracellular matrix was only observed on HA1250 and TCP, and its subsequent resorption by osteoclastlike cells was evident. Small and occasionally larger tartrate-resistant acid phosphatase positive cells produced 20-150 mu m diameter resorption pits in both the mineralized extracellular matrix on HA1250 and TCP and the surfaces of HA600 and HA900. Resorption of the mineralized extracellular matrix on TCP also resulted in degradation of the underlying ceramic surface, mainly initiating from intergrain boundaries, whereas the surface of HA1250 remained unaltered. The results of this study clearly demonstrate that osteoclastic resorption of calcium phosphates is potentiated in postosteogenic culture conditions. A possible role for bone matrix constituents in cell-mediated resorption is hypothesized, whereas the occurrence of resorption seems to be mainly governed by the combined effects of material characteristics such as grain size and crystal structure. (C) 1994 John Wiley and Sons, Inc.