The Inhibitory Effects of Quaternary Ammonium Methacrylates on Soluble and Matrix-bound MMPs
The Inhibitory Effects of Quaternary Ammonium Methacrylates on Soluble and Matrix-bound MMPs
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DOI：
10.1177/0022034510389472
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发表时间：
2011-04-01
影响因子：
7.6
通讯作者：
Pashley, D. H.
中科院分区：
文献类型：
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作者：
Tezvergil-Mutluay, A.;Agee, K. A.;Pashley, D. H.
与牙本质结合的基质金属蛋白酶（MMPs）有助于牙本质粘合剂产生的混合层中胶原纤维的进行性降解。本研究评价了甲基丙烯酸季铵（QAM）与可溶性rhMMP-9和基质结合的内源性MMP模型的MMP抑制潜力。最初通过rhMMP-9比色测定筛选六种不同的QAM。对于基质结合的内源性基质金属蛋白酶，我们在含钙和锌的培养基（CM;对照）、氯己定或CM中的QAM中老化脱矿牙本质梁30天，以确定干质量损失和胶原蛋白肽溶解相对于基线水平的变化。QAMs对可溶性rhMMP-9的抑制作用在34%和100%之间变化。在CM中孵育的梁显示干质量减少29%（p < 0.05），而与QAM孵育的梁仅显示干质量损失0.2%-6%。从在CM中孵育的梁检测到显著更多的溶解胶原（p < 0.05）。可以得出结论，QAMs表现出与洗必泰相当的牙本质MMP抑制，但需要更高的浓度。
Matrix metalloproteinases (MMPs) bound to dentin contribute to the progressive degradation of collagen fibrils in hybrid layers created by dentin adhesives. This study evaluated the MMP-inhibiting potential of quaternary ammonium methacrylates (QAMs), with soluble rhMMP-9 and a matrix-bound endogenous MMP model. Six different QAMs were initially screened by a rhMMP-9 colorimetric assay. For the matrix-bound endogenous MMPs, we aged demineralized dentin beams for 30 days in calcium-and zinc-containing media (CM; control), chlorhexidine, or QAMs in CM to determine the changes in dry mass loss and solubilization of collagen peptides against baseline levels. The inhibitory effects of QAMs on soluble rhMMP-9 varied between 34 and 100%. Beams incubated in CM showed a 29% decrease in dry mass (p < 0.05), whereas beams incubated with QAMs showed only 0.2%-6% loss of dry mass. Significantly more solubilized collagen was detected from beams incubated in CM (p < 0.05). It is concluded that QAMs exhibited dentin MMP inhibition comparable with that of chlorhexidine, but required higher concentrations.