Autoregulatory Effect of Interleukin-10 on Proinflammatory Cytokine Production by Porphyromonas gingivalis Lipopolysaccharide-Tolerant Human Monocytes
Autoregulatory Effect of Interleukin-10 on Proinflammatory Cytokine Production by Porphyromonas gingivalis Lipopolysaccharide-Tolerant Human Monocytes
复制标题
Interleukin-10 对牙龈卟啉单胞菌脂多糖耐受性人单核细胞产生促炎细胞因子的自动调节作用
作者：
H. Shimauchi;T. Ogawa;K. Okuda;Y. Kusumoto;H. Okada
ABSTRACT Pretreatment of human peripheral blood monocytes with a very low concentration (0.1 ng/ml) of Porphyromonas gingivalislipopolysaccharides (LPS) resulted in a significant decrease of interleukin-6 (IL-6) production, but not IL-8 production, by restimulation of a high concentration (1 μg/ml) of the same LPS. In contrast, the same pretreatment with Escherichia coli LPS resulted in the enhanced production of both IL-6 and IL-8 after restimulation. The selective induction by P. gingivalis LPS tolerance of IL-6 production developed in a time-dependent manner during the primary culture. P. gingivalis LPS-pretreated cells were also refractory to a high-dose E. coli LPS restimulation in terms of IL-6 production. The expression of IL-6 mRNA decreased 10 h after restimulation of P. gingivalisLPS-pretreated monocytes. Furthermore, an up-regulation of anti-inflammatory cytokine IL-10 upon a second high-dose LPS rechallenge occurred at the same time point in the pretreated cells. We studied the role of IL-10 in the process of IL-6 down-regulation. Neutralization by an anti-IL-10 polyclonal antibody prevented IL-6 down-regulation in P. gingivalis LPS-pretreated monocytes, whereas IL-8 production was not affected. Addition of exogenous IL-10 during the high-dose LPS stimulation of untreated cells substituted for the LPS pretreatment and resulted in the inhibition of IL-6 production in a dose-dependent manner. A higher dose of IL-10 was required to suppress IL-8 synthesis from monocytes. Our data suggest that IL-10 mediates IL-6 down-regulation in P. gingivalis LPS-tolerant monocytes in an autocrine manner.
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影响因子：
4.4
作者：
N. Mukaida;M. Shiroo;K. Matsushima
通讯作者：
N. Mukaida;M. Shiroo;K. Matsushima
脂多糖引发的巨噬细胞的重编程是通过 IL-10 和 IL-12 的平衡产生来控制的。
DOI：
--
发表时间：
1998
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Shnyra,A;Brewington,R;Alipio,A;Amura,C;Morrison,DC
通讯作者：
Morrison,DC
DOI：
10.1016/s0021-9258(18)50091-0
发表时间：
1992-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Bogdan;J. Paik;Y. Vodovotz;C. Nathan
通讯作者：
C. Bogdan;J. Paik;Y. Vodovotz;C. Nathan
IL-1 受体拮抗剂和人单核细胞中 IL-1 β 的产生受到不同的调节。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Arend,WP;SmithJr,MF;Janson,RW;Joslin,FG
通讯作者：
Joslin,FG
牙龈拟杆菌和中间拟杆菌识别人纤维蛋白原上的不同位点。
影响因子：
3.2
作者：
Lantz,MS;Allen,RD;Bounelis,P;Switalski,LM;Hook,M
通讯作者：
Hook,M