Intradiscal injections of osteogenic protein-1 restore the viscoelastic properties of degenerated intervertebral discs.
Intradiscal injections of osteogenic protein-1 restore the viscoelastic properties of degenerated intervertebral discs.
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DOI：
10.1016/j.spinee.2006.04.014
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发表时间：
2006-11-01
期刊：
影响因子：
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通讯作者：
An, Howard S
中科院分区：
文献类型：
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作者：
Miyamoto, Kei;Masuda, Koichi;An, Howard S
背景情况：在椎间盘（IVD）退变的兔模型中使用生物化学、组织学和放射学参数，椎间盘内注射生长因子（如成骨蛋白-1（OP-1））已显示可再生IVD。然而，很少有人知道这种生物治疗方法如何影响退化的IVD.Purpose的生物力学性能：研究椎间盘内注射OP-1对兔环形穿刺椎间盘退变模型中IVDs生物力学性能的影响，并确定它们与生物化学性能的关系。在兔环形穿刺椎间盘退变模型中，椎间盘内给予OP-1对IVD生物力学和生物化学特性影响的体内研究。新西兰白色家兔（n=16）在L2-L3和L4-L5（L3-L4：未穿刺对照）使用18号针进行纤维环（AF）穿刺。四周后，穿刺的椎间盘接受5%乳糖（10微升）或OP-1（100微克/10微升5%乳糖）注射到髓核（NP）中。每两周通过X线片监测椎间盘高度。注射后8周处死并取出骨-椎间盘-骨复合体后，通过在6个加载频率（0.05至2 Hz）下单轴压缩中施加一个正弦应变周期来测试IVD的动态粘弹性。结果：与注射乳糖的椎间盘相比，单次注射OP-1可显著恢复椎间盘高度（OP-1 vs.乳糖，p
BACKGROUND CONTEXT: Using biochemical, histological, and radiological parameters in a rabbit model of intervertebral disc (IVD) degeneration, the intradiscal injection of a growth factor, such as osteogenic protein-1 (OP-1), has been shown to regenerate the IVD. However, very little is known about how such a biological therapeutic approach affects the biomechanical properties of the degenerated IVD.PURPOSE: To investigate the effects of an intradiscal injection of OP-1 on the biomechanical properties of IVDs in the rabbit annular-puncture disc degeneration model and to determine their relationship to biochemical properties.STUDY DESIGN/SETTING: In vivo study on the effects of intradiscally administered OP-1 on the biomechanical and biochemical properties of IVDs in the rabbit annular-puncture disc degeneration model.METHODS: New Zealand White rabbits (n=16) underwent annulus fibrosus (AF) puncture, using an 18-gauge needle, at L2-L3 and L4-L5 (L3-L4: nonpunctured control). Four weeks later, the punctured discs received an injection of either 5% lactose (10 microL) or OP-1 (100 microg/10 microL of 5% lactose) into the nucleus pulposus (NP). The disc height was radiographically monitored biweekly. After sacrifice and removal of bone-disc-bone complexes 8 weeks postinjection, the dynamic viscoelastic properties of the IVDs were tested by applying a cycle of sinusoidal strain in uniaxial compression at six loading frequencies (0.05 to 2 Hz). The biochemical properties of the dissected IVDs were then analyzed and correlated with the biomechanical properties.RESULTS: A single injection of OP-1 significantly restored disc height when compared with the lactose-injected discs (OP-1 vs. lactose, p