Effects of Oxidized Pyrenes on the Biological Responses in the Human Bronchial Epithelial Cells
Effects of Oxidized Pyrenes on the Biological Responses in the Human Bronchial Epithelial Cells
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DOI：
10.3390/app12199664
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发表时间：
2022-10-01
影响因子：
2.7
通讯作者：
Takano, Hirohisa
中科院分区：
文献类型：
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作者：
Honda, Akiko;Inoue, Ken-ichiro;Takano, Hirohisa
虽然多环芳烃（PAHs）是有毒的，氧化的多环芳烃对健康和生物反应的影响仍然不清楚。在这项研究中，我们研究了不同浓度的芘（一种PAH）及其醌形式（即4，5-芘醌（PyQ）和1，8-PyQ +1，6-PyQ）对人肺上皮细胞（BEAS-2B）的体外影响。我们评估了细胞活力、细胞凋亡以及白细胞介素（IL）-6、IL-8、可溶性细胞间粘附分子-1（sICAM-1）和活性氧簇（ROS）的产生。暴露于1 μ M 4，5-PyQ或1，8-PyQ +1，6-PyQ增加细胞活性。在3 μ M时，与对照组相比，4，5-PyQ增加了晚期凋亡和/或坏死细胞的数量，而1，8-PyQ +1，6-PyQ增加了死亡细胞的数量。暴露于10 μ M的4，5-PyQ降低IL-6的产生，暴露于3或10 μ M的4，5-PyQ和1，8-PyQ +1，6-PyQ两者降低IL-8的产生。在10 μ M的1，8-PyQ +1，6-PyQ暴露后sICAM-1的产生增加。在细胞存在下，4，5-PyQ和1，8-PyQ +1，6-PyQ以浓度依赖性方式显著增加ROS产生;在无细胞的情况下，用1，8-PyQ +1，6-PyQ观察到类似的结果。总的来说，我们的研究结果表明，氧化多环芳烃诱导更强的呼吸毒性/炎症反应比多环芳烃。
Although polycyclic aromatic hydrocarbons (PAHs) are toxic, the effects of oxidized PAHs on health and biological responses remain unclear. In this study, we examined the in vitro effects of varying concentrations of pyrene, a type of PAH, and its quinone forms, namely 4,5-pyrenequinone (PyQ) and 1,8-PyQ + 1,6-PyQ, on human lung epithelial (BEAS-2B) cells. We evaluated cell viability, apoptosis, and the production of interleukin (IL)-6, IL-8, soluble intercellular adhesion molecule-1 (sICAM-1), and reactive oxygen species (ROS). Exposure to 1 mu M 4,5-PyQ or 1,8-PyQ + 1,6-PyQ increased the cellular activity. At 3 mu M, 4,5-PyQ increased the number of late apoptotic and/or necrotic cells compared with those in the control, whereas 1,8-PyQ + 1,6-PyQ increased the number of dead cells. Exposure to 4,5-PyQ at 10 mu M decreased IL-6 production and exposure to both 4,5-PyQ and 1,8-PyQ + 1,6-PyQ at 3 or 10 mu M decreased IL-8 production. sICAM-1 production was increased after 1,8-PyQ + 1,6-PyQ exposure at 10 mu M. In the presence of cells, 4,5-PyQ and 1,8-PyQ + 1,6-PyQ increased ROS production significantly in a concentration-dependent manner; similar results were observed with 1,8-PyQ + 1,6-PyQ without cells. Overall, our results suggest that oxidized PAHs induce stronger respiratory toxicity/inflammatory responses than PAHs.