Listeriosis in the pregnant guinea pig: a model of vertical transmission
Listeriosis in the pregnant guinea pig: a model of vertical transmission
复制标题
DOI：
10.1128/iai.72.1.489-497.2004
复制
发表时间：
2004-01-01
影响因子：
3.1
通讯作者：
Portnoy, DA
中科院分区：
文献类型：
--
作者：
Bakardjiev, AI;Stacy, BA;Portnoy, DA
胎儿-胎盘感染是妊娠并发症的主要原因，但垂直传播的潜在分子和细胞机制知之甚少。单核细胞增生李斯特菌是一种兼性细胞内病原体，是已知引起人类和其他哺乳动物胎儿-胎盘感染的一组病原体之一。本研究的目的是评价可能的垂直传播机制的L。单核细胞增多症。人类和豚鼠都有血绒膜胎盘，其中单层胎儿来源的滋养层将母体和胎儿的循环分开。我们刻画了L.在妊娠豚鼠模型和原代人滋养层细胞和滋养层细胞衍生细胞系中观察到胎儿-胎盘单位的单核细胞增多症感染。本文报道了豚鼠妊娠期肺吸虫病的临床表现及嗜性。豚鼠胎盘单核细胞增多症与人类相似。滋养层细胞培养系统允许细胞生长和细胞间扩散，并显示L。单核细胞增多症缺乏介导非吞噬细胞侵袭的毒力因子--内化素A，其侵袭力是缺乏的100倍。然而，在豚鼠模型中，胎儿-胎盘屏障的穿越不依赖于内分泌蛋白A，这表明内分泌蛋白介导的滋养层细胞在体内的直接侵袭作用可以忽略不计。对L.单核细胞增多症将有助于设计更有效的治疗和疾病预防手段。
Feto-placental infections represent a major cause of pregnancy complications, and yet the underlying molecular and cellular mechanisms of vertical transmission are poorly understood. Listeria monocytogenes, a facultative intracellular pathogen, is one of a group of pathogens that are known to cause feto-placental infections in humans and other mammals. The purpose of this study was to evaluate possible mechanisms of vertical transmission of L. monocytogenes. Humans and guinea pigs have a hemochorial placenta, where a single layer of fetally derived trophoblasts separates maternal from fetal circulation. We characterized L. monocytogenes infection of the feto-placental unit in a pregnant guinea pig model and in primary human trophoblasts and trophoblast-derived cell lines. The clinical manifestations of listeriosis in the pregnant guinea pigs and the tropism of L. monocytogenes to the guinea pig placenta resembled those in humans. Trophoblast cell culture systems were permissive for listerial growth and cell-to-cell spread and revealed that L. monocytogenes deficient in internalin A, a virulence factor that mediates invasion of nonphagocytic cells, was 100-fold defective in invasion. However, crossing of the feto-placental barrier in the guinea pig model was independent of internalin A, suggesting a negligible role for internalin-mediated direct invasion of trophoblasts in vivo. Further understanding of vertical transmission of L. monocytogenes will help in designing more effective means of treatment and disease prevention.