Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype
Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype
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DOI：
10.1016/j.tripleo.2010.03.009
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发表时间：
2010-08-01
期刊：
影响因子：
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通讯作者：
Kawakami, Toshiyuki
中科院分区：
文献类型：
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作者：
Siar, Chong Huat;Nagatsuka, Hitoshi;Kawakami, Toshiyuki
客观的。 Notch信号传导与牙发生和一些牙源性肿瘤的肿瘤发生过程中的细胞命运决定有关。然而，其在实性/多囊性（SA）、单囊性（UA）和复发性（RA）成釉细胞瘤中的作用仍不清楚。本研究的目的是确定这些亚型中Notch受体和配体的表达并推测其意义。方法。对 SA (n = 23)、UA (n = 22) 和 RA (n = 19) 中的 Notch 受体 (Notch1、2、3、4) 和配体 (Jagged1、2 和 Delta1) 进行免疫组织化学检查。结果。与 UA (n = 1/22; 4.5%) 或 RA (n = 10/19; 52.6%) 相比，SA (n = 19/23; 82.6%) 中 Notch4 过表达 (P < .05) 表明 Notch4 信号传导与实性/多囊性表型成釉细胞瘤之间呈正相关。配体（Jagged1 和 Delta1）与其受体（Notch1、3、4）相比表达不足（P < .05），并且所有 3 个亚群中 Notch2 和 Jagged2 均无反应性，表明成釉细胞瘤上皮属于谱系定型之前的早期分化阶段（相当于发育中牙胚的内釉质上皮）。结论。目前的研究结果表明，Notch 信号分子可能在不同成釉细胞瘤表型的获得中发挥不同的作用。 (口腔外科、口腔医学、口腔病理、口腔放射学 Endod 2010；110：224-233)
Objective. Notch signaling has been implicated in cell fate decisions during odontogenesis and tumorigenesis of some odontogenic neoplasms; however, its role in solid/multicystic (SA), unicystic (UA), and recurrent (RA) ameloblastoma remains unclear. The aim of this study was to determine Notch receptor and ligand expressions in these subtypes and to speculate on their significance.Methods. Notch receptors (Notch1, 2, 3, 4) and ligands (Jagged1, 2, and Delta1) were examined immunohistochemically in SA (n = 23), UA (n = 22), and RA (n = 19).Results. Notch4 overexpression in SA (n = 19/23; 82.6%) compared with UA (n = 1/22; 4.5%) or RA (n = 10/19; 52.6%) (P < .05) suggests positive correlation between Notch4 signaling and ameloblastomas with a solid/multicystic phenotype. Ligand (Jagged1 and Delta1) underexpression compared with their receptors (Notch1, 3, 4) (P < .05) and nonreactivity for Notch2 and Jagged2 in all 3 subsets suggests that ameloblastoma epithelium belongs to an earlier stage of differentiation (equivalent to inner enamel epithelium of developing tooth germ) before lineage commitment.Conclusion. Present findings suggest that Notch signaling molecules may play differing roles in the acquisition of different ameloblastoma phenotypes. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 110: 224-233)