Inhibition of HIV-1 gene expression by a fragment of hnRNP U.
Inhibition of HIV-1 gene expression by a fragment of hnRNP U.
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DOI：
10.1016/j.molcel.2006.07.021
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发表时间：
2006-08
期刊：
影响因子：
16
通讯作者：
S. Valente;S. Goff
中科院分区：
文献类型：
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作者：
S. Valente;S. Goff
细胞蛋白现在被认为在人类免疫缺陷病毒1型（HIV-1）生命周期的所有步骤中都起着至关重要的作用，破坏病毒复制所必需的宿主功能可能提供新的抗病毒方法。从人类互补DNA （cDNA）文库中选择能够诱导对重组HIV-1基因组感染产生抗性的克隆，结果鉴定出一个有效限制HIV-1活性的基因片段。活性cDNA编码异质核核糖核蛋白U （hnRNP U）的n端片段。该基因片段特异性靶向病毒mRNA中的3 ‘长末端重复序列（3 ’ ltr），并阻断HIV-1 mRNA的细胞质积累。结果表明，HIV-1需要能够被干扰基因特异性阻断的病毒mrna核输出机制。
Cellular proteins are now appreciated as critically involved in all steps of the human immunodeficiency virus type 1 (HIV-1) life cycle, and disrupting host functions essential for virus replication may provide novel antiviral approaches. Selection from a human complementary DNA (cDNA) library for clones able to induce resistance to infection by recombinant HIV-1 genomes resulted in the identification of a gene fragment that potently restricts HIV-1 activity. The active cDNA encodes an N-terminal fragment of the heterogeneous nuclear ribonuclear protein U (hnRNP U). The gene fragment specifically targets the 3′ long terminal repeat (3′LTR) in the viral mRNA and blocks the cytoplasmic accumulation of HIV-1 mRNAs. The results suggest that HIV-1 requires machinery for the nuclear export of viral mRNAs that can be specifically blocked by an interfering gene.