A novel device detects a rapid ozone-induced transient stomatal closure in intact Arabidopsis and its absence in abi2 mutant
A novel device detects a rapid ozone-induced transient stomatal closure in intact Arabidopsis and its absence in abi2 mutant
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DOI：
10.1111/j.1399-3054.2006.00851.x
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发表时间：
2007-04-01
影响因子：
6.4
通讯作者：
Kollist, Hannes
中科院分区：
文献类型：
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作者：
Kollist, Triin;Moldau, Heino;Kollist, Hannes
为了跟踪不同拟南芥品系气孔对臭氧（O-3）的响应，我们构建了一个快速响应的O-3暴露/气体交换测量装置，该装置由8个通流全莲座比色皿组成。为了将莲座丛与根和生长基质分离，植物通过固定在盆上的抛光玻璃板上的琼脂填充的孔生长。在插入植物之后，板形成比色皿的气密底表面;因此，在将植物插入比色皿的任何阶段期间，莲座被封闭而不接触它。该装置允许监测气孔功能的快速反应。例如，急性暴露于150 ppb的O-3降低气孔导度的60-70%，其初始值在9-12分钟。此后，电导恢复其暴露前的值在进一步的30-40分钟内，尽管继续O-3曝光。脱落酸不敏感突变体abi 2在2C类蛋白磷酸酶缺陷的瞬时减少是不存在的。这提供了一个在体内确认气孔导度的早期瞬时降低是不是由O-3分解形成的活性氧（ROS）的物理损伤的结果，但反映了ROS的生物学作用，通过信号转导级联。因此，该装置将有助于在体内指定复杂的分子和遗传相互作用的快速反应中的保卫细胞。
To follow stomatal responses to ozone (O-3) in different Arabidopsis lines, we constructed a rapid-response O-3 exposure/gas-exchange measurement device consisting of eight through-flow whole-rosette cuvettes. To separate rosette from roots and growth substrate, plant is grown through an agar-filled hole in a polished glass plate fixed on the pot. Following insertion of the plant, the plate forms air-tight bottom surface of the cuvette; thus the rosette is enclosed without touching it during any phase of the insertion of the plant to the cuvette. The device allows monitoring rapid responses in the stomatal function. For example, an acute exposure to 150 ppb O-3 decreased stomatal conductance to 60-70% of its initial value within 9-12 min. Thereafter, the conductance regained its preexposure value within further 30-40 min in spite of the continuing O-3 exposure. The transient decrease was absent in the abscisic acid-insensitive mutant abi2 defective in a class 2C protein phosphatase. This provides an in vivo confirmation that the early transient decrease in stomatal conductance is not a result of physical damage by the reactive oxygen species (ROS) formed from O-3 breakdown but reflects the biological action of ROS, transduced through a signalling cascade. Thus, the apparatus will be helpful in specifying complex molecular and genetic interactions in rapid responses in guard cells in vivo.