A long non-coding RNA, PTCSC3, as a tumor suppressor and a target of miRNAs in thyroid cancer cells.
A long non-coding RNA, PTCSC3, as a tumor suppressor and a target of miRNAs in thyroid cancer cells.
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DOI：
10.3892/etm.2013.933
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发表时间：
2013-04
影响因子：
2.7
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Fan M;Li X;Jiang W;Huang Y;Li J;Wang Z
甲状腺乳头状癌候选基因3(PTCSC3)是新近发现的一种高度甲状腺特异性的非编码RNA。甲状腺癌中的显著下调表明它在甲状腺肿瘤的发生和发展中具有潜在的作用。本研究旨在研究PTCSC3对甲状腺癌细胞生物学特性的影响，并探讨其作为竞争内源性RNA与miRNAs结合的可能功能。将含有长非编码RNA的构建体PTCSC3导入不同的甲状腺癌细胞系(bCPAP、FTC133和8505C)。用四甲基偶氮唑盐比色法和流式细胞仪检测细胞生长、细胞周期变化和细胞凋亡。在电子计算机分析中确定PTCSC3与靶miRNAs的结合位点。此外，通过定量逆转录聚合酶链式反应(RT-PCR)检测PTCSC3转染的甲状腺癌细胞中可能存在的miRNA，以证实这种相互作用。PTCSC3基因转染后，来自不同病理类型甲状腺癌的三种甲状腺癌细胞均表现出明显的生长抑制、细胞周期停滞和细胞凋亡增加。筛选出与PTCSC3有潜在相互作用的前20个miRNAs，从中筛选出miR-574-5p，进一步证实了在体外甲状腺癌细胞中与PTCSC3的负相关。在本研究中，PTCSC3作为一种肿瘤抑制因子被研究为miR-574-5p的竞争内源RNA。
Papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) is a newly identified non-coding RNA, which is highly thyroid-specific. Dramatic downregulation in thyroid cancers suggests its potential roles in the occurrence and development of thyroid tumors. The present study aimed to investigate the effects of PTCSC3 on the biological features of thyroid cancer cells and to explore its possible function as a competing endogenous RNA to bind with miRNAs. Constructs containing the long non-coding RNA, PTCSC3, were transfected into various thyroid cancer cell lines (BCPAP, FTC133 and 8505C). Cell growth, cell cycle transition and apoptosis were measured by MTT assay and flow cytometry. In silico analysis was performed to identify the binding site of PTCSC3 for target miRNAs. Additionally, detection of putative miRNA by quantitative reverse transcription-polymerase chain reaction (RT-PCR) in thyroid cancer cells transfected with PTCSC3 was determined to confirm the interaction. Following transfection with PTCSC3, all three thyroid cancer cells originating from various pathological types of thyroid cancers demonstrated significant growth inhibition, cell cycle arrest and increased apoptosis. The top 20 miRNAs to have a potential interaction with PTCSC3 were identified, out of which miR-574-5p was selected to further confirm the inverse correlation with PTCSC3 in thyroid cancer cells in vitro. In the present study, PTCSC3 as a tumor suppressor was investigated as a competing endogenous RNA for miR-574-5p.
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