Embryonic expression of an Nkx2-5/Cre gene using ROSA26 reporter mice
Embryonic expression of an Nkx2-5/Cre gene using ROSA26 reporter mice
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DOI：
10.1002/gene.10022
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发表时间：
2001-12-01
期刊：
影响因子：
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通讯作者：
Schwartz, Robert J.
中科院分区：
文献类型：
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作者：
Moses, Kelvin A.;DeMayo, Franco;Schwartz, Robert J.
Nkx2 - 5是脊椎动物胚胎中最早的心脏特异性标志物之一，被用作一个基因位点，通过同源重组敲入Cre重组酶基因。杂合的Nkx2 - 5/Cre小鼠与ROSA26（R26R）报告小鼠交配产生的后代，为通过β - 半乳糖苷酶（β - gal）活性追踪Nkx2 - 5基因活性提供了一个模型系统。β - gal活性最初在心脏新月早期、环化心管的心肌细胞以及第一咽弓的上皮细胞中被观察到。在后期胚胎（交配后10.5 - 13.5天，dpc）中，β - gal活性在胃和脾脏、舌背以及牙齿的凝聚原基中被观察到。Nkx2 - 5/Cre小鼠模型应该提供一种有用的遗传资源，以阐明受loxP操纵的基因靶点在心脏发生和其他发育过程中的作用。
Nkx2-5, one of the earliest cardiac-specific markers in vertebrate embryos, was used as a genetic locus to knock in the Cre recombinase gene by homologous recombination. Offspring resulting from heterozygous Nkx2-5/Cre mice mated to ROSA26 (R26R) reporter mice provided a model system for following Nkx2-5 gene activity by beta-galactosidase (beta-gal) activity. beta-gal activity was initially observed in the early cardiac crescent, cardiomyocytes of the looping heart tube, and in the epithelium of the first pharyngeal arch. In later stage embryos (10.5-13.5 days postcoitum, dpc), beta-gal activity was observed in the stomach and spleen, the dorsum of the tongue, and in the condensing primordium of the tooth. The Nkx2-5/Cre mouse model should provide a useful genetic resource to elucidate the role of loxP manipulated genetic targets in cardiogenesis and other developmental processes.