Iron oxide nanoparticles in liquid or powder form enhanced osteogenesis via stem cells on injectable calcium phosphate scaffold.
Iron oxide nanoparticles in liquid or powder form enhanced osteogenesis via stem cells on injectable calcium phosphate scaffold.
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DOI：
10.1016/j.nano.2019.102069
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发表时间：
2019-10
期刊：
影响因子：
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通讯作者：
Yang Xia;Yantao Zhao;Feimin Zhang;Bo Chen;Xiantong Hu;M. Weir;A. Schneider;Lu Jia;N. Gu
中科院分区：
文献类型：
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作者：
Yang Xia;Yantao Zhao;Feimin Zhang;Bo Chen;Xiantong Hu;M. Weir;A. Schneider;Lu Jia;N. Gu
本研究的目的是将纳米氧化铁(IONPs)引入到磷酸钙骨水泥(CPC)中以增强骨工程，并首次研究IONPs作为液体或粉末对使用IONP-CPC支架的干细胞的影响。以液体或粉末形式加入1%的IONPs制备IONP-CPCs。将人牙髓干细胞(HDPSCs)接种。对小鼠皮下埋植进行了研究。与CPC对照组相比，IONP-CPC具有更好的细胞铺展能力、更强的碱性磷酸酶活性和骨矿合成能力。皮下植入6周后，各组均表现出良好的生物相容性。综上所述，在IONP-CPC支架上以液体或粉末形式引入IONPs均能显著增强hDPSCs，并表现出良好的生物相容性。IONP液体掺入较IONP粉剂更能促进人DPSC向成骨方向分化。在CPC中，IONPs和壳聚糖乳酸盐联合使用比单独使用更能促进hDPSCs的成骨。
The objectives of this study were to incorporate iron oxide nanoparticles (IONPs) into calcium phosphate cement (CPC) to enhance bone engineering, and to investigate the effects of IONPs as a liquid or powder on stem cells using IONP-CPC scaffold for the first time. IONP-CPCs were prepared by adding 1% IONPs as liquid or powder. Human dental pulp stem cells (hDPSCs) were seeded. Subcutaneous implantation in mice was investigated. IONP-CPCs had better cell spreading, and greater ALP activity and bone mineral synthesis, than CPC control. Subcutaneous implantation for 6 weeks showed good biocompatibility for all groups. In conclusion, incorporating IONPs in liquid or powder form both substantially enhanced hDPSCs on IONP-CPC scaffold and exhibited excellent biocompatibility. IONP incorporation as a liquid was better than IONP powder in promoting osteogenic differentiation of hDPSCs. Incorporating IONPs and chitosan lactate together in CPC enhanced osteogenesis of hDPSCs more than using either alone.