A dual role of amino acids from Sesbania rostrata seed exudates in the chemotaxis response of Azorhizobium caulinodans ORS571
A dual role of amino acids from Sesbania rostrata seed exudates in the chemotaxis response of Azorhizobium caulinodans ORS571
复制标题
田菁种子分泌物氨基酸在固氮根瘤菌 ORS571 趋化反应中的双重作用
DOI：
10.1094/mpmi-03-19-0059-r
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发表时间：
2019
影响因子：
3.5
通讯作者：
Haishuan Sun
中科院分区：
文献类型：
--
作者：
Xiaolin Liu;Zhihong Xie;Yixuan Wang;Yu Sun;Xiaoxiao Dang;Haishuan Sun
固氮根瘤菌ORS571能在其寄主田菁的根和茎上诱导结瘤。植物分泌物在微生物与寄主植物之间的对话中是必不可少的，尤其是氨基酸在细菌的趋化反应中可以发挥重要作用。组氨酸、精氨酸和天冬氨酸是胰岛素中含量最丰富的三种氨基酸。玫瑰花籽分泌物，对A有化学引诱剂的作用。根茎类。OFA基因组中甲基接受趋化蛋白(MCPs)(趋化受体)的位置特异性迭代BLAST分析。行髓鞘切断术。在43个MCP同源物中，两个含有dCache域的MCP被选为可能的同源氨基酸MCP。经过对相关基因表达水平的分析和基因缺失突变株的构建，其中一株被设计为T1pH的突变株AZC_0821被证实对这三种氨基酸产生趋化反应。此外，还发现这三种氨基酸对OFA的趋化性也有影响。不依赖于化学感觉受体，通过参与参与趋化途径和鞭毛合成的几种化学和鞭毛烯的表达水平的增加而独立于化学感觉受体。因此，种子分泌物中存在的氨基酸的贡献与其作为趋化物质的作用直接相关，也间接地与参与趋化和运动的关键基因的表达调控有关。这种“双重角色”很可能会影响生物膜的形成。木聚糖硫酸酯及其寄主根定植特性。
Azorhizobium caulinodansORS571 can induce nodule formation on the roots and the stems of its host legume,Sesbania rostrata. Plant exudates are essential in the dialogue between microbes and their host plant and, in particular, amino acids can play an important role in the chemotactic response of bacteria. Histidine, arginine, and aspartate, which are the three most abundant amino acids present inS. rostrataseed exudates, behave as chemoattractants towardA. caulinodans. A position-specific-iterated BLAST analysis of the methyl-accepting chemotaxis proteins (MCPs) (chemoreceptors) in the genome ofA. caulinodanswas performed. Among the 43 MCP homologs, two MCPs harboring a dCache domain were selected as possible cognate amino acid MCPs. After analysis of relative gene expression levels and construction of a gene-deleted mutant strain, one of them, AZC_0821 designed as TlpH, was confirmed to be responsible for the chemotactic response to the three amino acids. In addition, it was found that these three amino acids can also influence chemotaxis ofA. caulinodansindependently of the chemosensory receptors, by being involved in the increase of the expression level of severalcheandflagenes involved in the chemotaxis pathway and flagella synthesis. Thus, the contribution of amino acids present in seed exudates is directly related to the role as chemoattractants and indirectly related to the role in the regulation of expression of key genes involved in chemotaxis and motility. This “dual role” is likely to influence the formation of biofilms byA. caulinodansand the host root colonization properties of this bacterium.