MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA CODING FOR THE PRECURSOR OF THE HUMAN CYSTEINE PROTEINASE-INHIBITOR CYSTATIN-C
MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA CODING FOR THE PRECURSOR OF THE HUMAN CYSTEINE PROTEINASE-INHIBITOR CYSTATIN-C
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DOI：
10.1016/0014-5793(87)80695-6
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发表时间：
1987-06-01
期刊：
影响因子：
3.5
通讯作者：
LUNDWALL, A
中科院分区：
文献类型：
--
作者：
ABRAHAMSON, M;GRUBB, A;LUNDWALL, A
从人胎盘 λgt11 cDNA 文库中分离出重组半胱氨酸蛋白酶抑制剂 C 产生克隆。其中一个克隆的 cDNA 插入片段包含 777 个碱基对，编码完整的成熟胱抑素 C（120 个氨基酸）和 26 个氨基酸的疏水前导序列，表明该抑制剂具有细胞外功能。推导的蛋白质序列证实了从人尿中分离的半胱氨酸蛋白酶抑制剂C的蛋白质序列，但与遗传性脑出血伴淀粉样变性中作为淀粉样蛋白沉积的半胱氨酸蛋白酶抑制剂C片段的序列在一个位置上不同。
Recombinant cystatin C producing clones were isolated from a human placenta λgt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.