MULTIPLE PATERNITY AND SELF-FERTILIZATION IN RELATION TO FLORAL AGE IN MIMULUS GUTTATUS (SCROPHULARIACEAE)
MULTIPLE PATERNITY AND SELF-FERTILIZATION IN RELATION TO FLORAL AGE IN MIMULUS GUTTATUS (SCROPHULARIACEAE)
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DOI：
10.2307/2444854
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发表时间：
1991-12-01
影响因子：
3
通讯作者：
RITLAND, K
中科院分区：
文献类型：
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作者：
DUDASH, MR;RITLAND, K
为了获得一些了解的机制，负责的中间水平的多个父亲和自交先前观察到的共同monkeyflower，Mimulus guttatus，我们进行了一个田间试验，以评估1）多个父亲通过顺序授粉访问和2）通过花冠脱落自交的作用。In M.花开放数天后，在花冠脱落的过程中，花药从柱头上拖过去。我们预测，在此期间，传粉者的多次访问应增加多个父系的兄弟姐妹阵列的程度，花冠拖动可能是观察到的自交的主要原因。在三个北方加州人口花冠被删除后，开放1天（防止进一步交配），交配的类型进行了比较，从相邻的花朵与未受干扰的花冠交配。平均而言，在花冠未经处理的花朵中，种子的结实率是其两倍，这表明在M。1天后出现点滴花。花粉补充表明，花粉是限制在一个人口。电泳分析表明，两个群体中未授粉的花多亲性较高，表明顺序授粉可能是多亲性的重要来源。然而，两种处理之间的自交率没有显着差异，表明即使在花粉限制条件下，花冠拖动也不是自交的来源。
To gain some understanding of the mechanisms responsible for the intermediate levels of both multiple paternity and selfing observed previously in the common monkeyflower, Mimulus guttatus, we performed a field experiment to assess the role of 1) multiple patemity through sequential pollinator visits and 2) selfing through corolla abscission. In M. guttatus, flowers remain open for several days; then, in the process of corolla abscission anthers drag past the stigma. We predicted that multiple visitation by pollinators over this period should increase the degree of multiple paternity of sibling arrays, and that corolla dragging may be the primary cause of the observed selfing. In three northern California populations corollas were removed after being open for 1 day (preventing further mating), and the type of matings resulting was compared to matings from adjacent flowers with undisturbed corollas. On average, seed set was twice as great in flowers with unmanipulated corollas, indicating that about half of all matings in the lifespan of a M. guttatus flower occur after 1 day. Pollen supplementation revealed that pollen was limiting in one population. Electrophoretic assay of progeny showed multiple patemity was greater in unmanipulated flowers in the two populations that were not pollen limited, suggesting that sequential pollination may be an important source of multiple patemity. However, no difference was detected in the selfing rate between the two treatments, suggesting that corolla dragging was not a source of selfing even under pollen-limited conditions.