A novel chloride channel localizes to Caenorhabditis elegans spermatids and chloride channel blockers induce spermatid differentiation
A novel chloride channel localizes to Caenorhabditis elegans spermatids and chloride channel blockers induce spermatid differentiation
复制标题
DOI：
10.1006/dbio.1996.9999
复制
发表时间：
1996-05-25
影响因子：
2.7
通讯作者：
LHernault, SW
中科院分区：
文献类型：
--
作者：
Machaca, K;DeFelice, LJ;LHernault, SW
秀丽隐杆线虫精子发生特别适合研究细胞分化过程中的非随机细胞质分离。精母细胞从无核的胞质核心中分离出来，并经历两次连续的分裂。在第二次分裂过程中，当精子细胞从无核残留体中出芽时，细胞内的细胞器特异性地分离为精子细胞。我们应用膜片钳技术，以调查膜蛋白分布在这些不对称分裂。我们发现，膜组件，电压依赖性离子通道活性测定，遵循特定的分布模式在精子发育过程中。在精母细胞和残留体中观察到几种电压敏感离子通道活性，但在精子细胞中只能检测到单通道类型，这表明其他通道活性在这些细胞形成时被排除或失活。在精子细胞中观察到的通道是内向整流氯离子通道（Clir），如其对氯离子通道抑制剂的敏感性和其电导的Cl依赖性变化所示。用Cl通道阻断剂处理精子细胞诱导其分化为精子，表明Clir在这一发育步骤中起作用。这些研究是膜片钳电生理学在C. elegans发育(C)出版社：Academic Press，Inc.
Caenorhabditis elegans spermatogenesis is especially suited for studies of nonrandom cytoplasmic segregation during cellular differentiation. Spermatocytes separate from an anuclear cytoplasmic core and undergo two sequential divisions. During the second division, intracellular organelles segregate specifically to spermatids as they bud from an anuclear residual body. We have applied patch-clamp techniques in order to investigate membrane protein distribution during these asymmetric divisions. We show that membrane components, as assayed by voltage-dependent ion channel activity, follow a specific distribution pattern during sperm development. Several voltage-sensitive ion channel activities are observed in spermatocytes and residual bodies, but only a single-channel type can be detected in spermatids, indicating that other channel activities are excluded from or inactivated within these cells as they form. The channel that is observed in spermatids is an inward-rectifying chloride channel (Clir), as indicated by its sensitivity to chloride channel inhibitors and Cl-dependent shifts in its conductance. Treatment of spermatids with Cl channel blockers induce their differentiation into spermatozoa, suggesting that Clir plays a role during this developmental step. These studies are the first application of patch-clamp electrophysiology to C. elegans development. (C) 1996 Academic Press, Inc.