Neurochemical coding of the enteric nervous system in chagasic patients with megacolon
Neurochemical coding of the enteric nervous system in chagasic patients with megacolon
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DOI：
10.1007/s10620-006-9680-5
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发表时间：
2007-10-01
影响因子：
3.1
通讯作者：
Furness, J. B.
中科院分区：
文献类型：
--
作者：
da Silveira, A. B. M.;Reis, D. D'Avila;Furness, J. B.
神经元破坏被认为是恰加斯巨结肠致病机制的标志。对巨结肠恰加斯病患者肠神经系统中神经肽的表征可以阐明该综合征发展的某些方面。在目前的工作中，我们证明了巨结肠恰加斯病患者结肠神经元丛中存在的神经肽和神经化学标记物表达的变化。对冷冻组织样本切片进行免疫组织化学标记，以检测抗视网膜素、cChaT、P 物质、VIP、NOS 和 NPY。使用共聚焦显微镜观察免疫反应性。我们的结果表明，在巨结肠患者中，抑制性运动神经元（VIP 和 NOS 免疫反应性）优先被克氏锥虫和/或炎症过程破坏。这些结果表明，患有巨结肠的恰加斯病患者结肠中的肠神经元受到选择性破坏，这表明恰加斯病发病机制的重要发现。
Neuronal destruction has been considered the hallmark of pathogenic mechanisms in chagasic megacolon. Characterization of neuropeptides in the enteric nervous system from chagasic patients with megacolon could elucidate some aspects of the development of this syndrome. In the present work we demonstrate the changes in expression of neuropeptides and neurochemical markers present in neuronal plexuses from the colons of chagasic patients with megacolon. Sections of frozen tissue samples were immunohistochemically labeled for anticalretinin, cChaT, substance P, VIP, NOS, and NPY. Immunoreactivity was observed using a confocal microscope. Our results demonstrate that in chagasic patients with megacolon, inhibitory motor neurons ( VIP and NOS immunoreactive) are preferentially destroyed by Trypanosoma cruzi and/or the inflammatory process. These results suggest a selective destruction of enteric neurons in the colon of chagasic patients with megacolon, pointing to an important discovery in the mechanism of pathogenesis of Chagas' disease.