Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples
Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples
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DOI：
10.3791/61309
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发表时间：
2020-10-01
影响因子：
1.2
通讯作者：
Boyko, Matthew
中科院分区：
文献类型：
--
作者：
Frank, Dmitry;Gruenbaum, Benjamin F.;Boyko, Matthew
缺血性中风是全世界最常见的发病和死亡原因之一。历史上，用于刺激缺血性卒中的动物模型涉及大脑中动脉闭塞（MCAO）。梗死区、脑水肿和血脑屏障（BBB）破坏作为反映MCAO后脑损伤程度的参数。这种方法的一个重要限制是，这些测量通常是在不同的大鼠大脑样本中获得的，由于需要对大量大鼠实施安乐死以获得适当的样本量，这导致了伦理和经济负担。本研究提出了一种通过测量同一组大鼠脑梗死区、脑水肿和血脑屏障通透性来准确评估MCAO后脑损伤的方法。这项新技术为评估脑卒中的病理生理提供了一种更有效的方法。
One of the most common causes of morbidity and mortality worldwide is ischemic stroke. Historically, an animal model used to stimulate ischemic stroke involves middle cerebral artery occlusion (MCAO). Infarct zone, brain edema and blood-brain barrier (BBB) breakdown are measured as parameters that reflect the extent of brain injury after MCAO. A significant limitation to this method is that these measurements are normally obtained in different rat brain samples, leading to ethical and financial burdens due to the large number of rats that need to be euthanized for an appropriate sample size. Here we present a method to accurately assess brain injury following MCAO by measuring infarct zone, brain edema and BBB permeability in the same set of rat brains. This novel technique provides a more efficient way to evaluate the pathophysiology of stroke.