Platelet‐activating factor and lyso‐PAF possess direct antimicrobial properties in vitro
Platelet‐activating factor and lyso‐PAF possess direct antimicrobial properties in vitro
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DOI：
10.1034/j.1600-0463.2002.100206.x
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发表时间：
2002-02
期刊：
影响因子：
2.8
通讯作者：
H. Steel;R. Cockeran;R. Anderson
中科院分区：
文献类型：
--
作者：
H. Steel;R. Cockeran;R. Anderson
在体外研究了浓度为0.25-20 μg/ml的血小板活化因子（PAF）和溶血血小板活化因子（L-PAF）对革兰氏阳性菌和革兰氏阴性菌钾转运和生长的影响，并与溶血磷脂酰胆碱（LPC）进行了比较。钾运输测定使用86 Rb+作为示踪剂，而生长测量根据放射性标记的氨基酸的摄取程度。所有试验磷脂均对革兰氏阳性菌的86 Rb+摄取和生长产生剂量相关抑制，效力顺序为PAF>LPC> L-PAF。另一方面，革兰氏阴性菌对磷脂对K+转运和生长的抑制作用不太敏感。一些（但不是全部）革兰氏阳性和革兰氏阴性细菌能够降解LPC，但不能降解PAF或L-PAF，这表明磷脂的酶促降解不能解释对这些试剂的不同敏感性。生物活性磷脂LPC、PAF和L-PAF可能代表主要针对革兰氏阳性菌的氧非依赖性抗微生物宿主防御系统。
The effects of platelet‐activating factor (PAF) and lyso‐platelet‐activating factor (L‐PAF) at concentrations of 0.25–20 μg/ml on potassium transport and growth of gram‐positive and gram‐negative bacteria have been investigated in vitro and compared with those of lysophosphatidylcholine (LPC). Potassium transport was determined using 86Rb+ as tracer, while growth was measured according to the extent of uptake of radiolabeled amino acids. All of the test phospholipids caused dose‐related inhibition of 86Rb+‐uptake and growth of gram‐positive bacteria, the order of potency being PAF>LPC>L‐PAF. Gram‐negative bacteria, on the other hand, were less sensitive to the inhibitory effects of the phospholipids on K+ transport and growth. Some, but not all, of the gram‐positive and gram‐negative bacteria were able to degrade LPC, but not PAF or L‐PAF, demonstrating that enzymatic degradation of phospholipids does not explain the differential sensitivity to these agents. The bioactive phospholipids LPC, PAF and L‐PAF may represent an oxygen‐independent antimicrobial host defense system operative primarily against gram‐positive bacteria.