Monte Carlo analysis of two-photon fluorescence imaging through a scattering medium
Monte Carlo analysis of two-photon fluorescence imaging through a scattering medium
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DOI：
10.1364/ao.37.008092
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发表时间：
1998-12-01
期刊：
影响因子：
1.9
通讯作者：
Saloma, C
中科院分区：
文献类型：
--
作者：
Blanca, CM;Saloma, C
利用蒙特卡罗方法分析了双光子荧光通过散射介质的成像行为。在不同的数值孔径和不同的散射深度与平均自由程的比值下，导出了各向同性和各向异性散射体在聚焦高斯光束中激发光子的轴向和横向分布，由归一化激发强度分布的平方确定了样品的双光子荧光分布。对于相同的透镜孔径和散射介质，双光子荧光成像比单光子共焦荧光成像提供更清晰和更少像差的轴向响应。相应的横向荧光分布中的对比度也显著更高。比较了各向同性和各向异性散射介质对共焦反射成像的影响。文中还讨论了蒙特卡罗模拟的收敛性。(C)1998年美国光学学会。
The behavior of two-photon fluorescence imaging through a scattering medium is analyzed by use of the Monte Carlo technique. The axial and transverse distributions of the excitation photons in the focused Gaussian beam are derived for both isotropic and anisotropic scatterers at different numerical apertures and at various ratios of the scattering depth with the mean free path. The two-photon fluorescence profiles of the sample are determined from the square of the normalized excitation intensity distributions. For the same lens aperture and scattering medium, two-photon fluorescence imaging offers a sharper and less aberrated axial response than that of single-photon confocal fluorescence imaging. The contrast in the corresponding transverse fluorescence profile is also significantly higher. Also presented are results comparing the effects of isotropic and anisotropic scattering media in confocal reflection imaging. The convergence properties of the Monte Carlo simulation are also discussed. (C) 1998 Optical Society of America.