Comparison of bacterial microbiota of the predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae) and its factitious prey Tyrophagus putrescentiae (Acari: Acaridae).
Comparison of bacterial microbiota of the predatory mite Neoseiulus cucumeris (Acari: Phytoseiidae) and its factitious prey Tyrophagus putrescentiae (Acari: Acaridae).
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DOI：
10.1038/s41598-017-00046-6
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发表时间：
2017-01-31
影响因子：
4.6
通讯作者：
Hubert J
中科院分区：
文献类型：
--
作者：
Pekas A;Palevsky E;Sumner JC;Perotti MA;Nesvorna M;Hubert J
黄瓜新小绥螨是一种捕食性螨类，用于节肢动物害虫的生物防治。大规模饲养的捕食者被喂食食腐食酪螨（Tyrophagus putrescentiae）。虽然对N. cucumeris和T.虽然腐殖菌存在，但尚不清楚这两个物种是否共享细菌群落。分析了捕食螨和被捕食螨种群中的细菌群落，以及潜在螨病原细菌的出现情况。比较基于以下组：（i）N.黄瓜批量生产;（ii）N.（iii）黄瓜炭疽病菌（T.腐殖菌纯种群;（iv）T.腐食线虫N.黄瓜属只有15%的OTU存在于来自捕食性和猎物螨种群的所有样品中（核心OTU）：细胞内共生体沃尔巴克氏体、Cardinium，加上其他Blattabacterium样、Solitalea样和Bartonella样共生体。环境细菌在捕食螨中含量较高，而共生细菌在捕食螨中含量较高。某些细菌类群的相对数量显着不同的微生物群的捕食螨饲养和不含N。黄瓜属健康N. cucumeris与N.黄瓜表现出疾病症状。我们没有在微生物群中鉴定出任何确认的螨病原菌。
Neoseiulus cucumeris is a predatory mite used for biological control of arthropod pests. Mass-reared predators are fed with factitious prey mites such as Tyrophagus putrescentiae. Although some information on certain endosymbionts of N. cucumeris and T. putrescentiae exists, it is unclear whether both species share bacterial communities. The bacterial communities in populations of predator and prey mites, as well as the occurence of potential acaropathogenic bacteria were analyzed. The comparisons were based on the following groups: (i) N. cucumeris mass-production; (ii) N. cucumeris laboratory population with disease symptoms; (iii) T. putrescentiae pure populations and; (iv) T. putrescentiae from rearing units of N. cucumeris. Only 15% of OTUs were present in all samples from predatory and prey mite populations (core OTUs): the intracellular symbionts Wolbachia, Cardinium, plus other Blattabacterium-like, Solitalea-like, and Bartonella-like symbionts. Environmental bacteria were more abundant in predatory mites, while symbiotic bacteria prevailed in prey mites. Relative numbers of certain bacterial taxa were significantly different between the microbiota of prey mites reared with and without N. cucumeris. No significant differences were found in the bacterial communities of healthy N. cucumeris compared to N. cucumeris showing disease symptoms. We did not identify any confirmed acaropathogenic bacteria among microbiota.