Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka
Expression of gonadal soma derived factor (GSDF) is spatially and temporally correlated with early testicular differentiation in medaka
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DOI：
10.1016/j.gep.2010.06.005
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发表时间：
2010-09-01
影响因子：
1.2
通讯作者：
Nagahama, Yoshitaka
中科院分区：
文献类型：
--
作者：
Shibata, Yasushi;Paul-Prasanth, Bindhu;Nagahama, Yoshitaka
在硬骨鱼，青（Ozyzias latipes），性别是在受精时由基因决定的。雄性为异配子体，染色体组成为XY，雌性为XX。青鳉XY胚胎的雄性分化是由性别决定基因Dmy启动的。在这项研究中，我们已经克隆了性腺索马衍生因子（GSDF）青鳉和其特征的表达模式在睾丸形态分化的启动。实时荧光定量PCR检测到Gsdf基因在青鳉受精后6 d（dpf）整个胚胎中XY特异性表达上调，与XY性腺睾丸分化的开始相一致。整体和切片原位杂交证实Gsdf仅在6 dpf遗传雄性的原始性腺中表达。相反，Gsdf的表达被发现是非常弱的XX性腺在胚胎发生。重要的是，发现Gsdf和Dmy共定位于XY性腺中的相同体细胞中。当用雌二醇-17 β处理XY胚胎时，为了逆转其表型性别，通过实时PCR观察到这些胚胎中Gsdf的表达下降。(C)2010 Elsevier B. V.保留所有权利。
In the teleost fish, medaka (Ozyzias latipes), the sex is genetically determined at the time of fertilization. The males are heterogametic with XY chromosome composition, while females are of XX chromosome composition. The male sexual differentiation is initiated in XY embryos of medaka by the sex-determining gene Dmy. In this study, we have cloned the gonadal soma derived factor (Gsdf) from medaka and characterized its expression pattern during the initiation of morphological testicular differentiation. By real-time PCR, an XY-specific up-regulation was detected in the expression levels of Gsdf in the whole embryos of medaka at 6 days post fertilization (dpf), coincident with the initiation of testicular differentiation in the XY gonads. Whole mount and section in situ hybridizations reaffirmed that Gsdf was expressed exclusively in primordial gonads of only the genetic males at 6 dpf. Conversely, the expression of Gsdf was found to be very weak in the XX gonads during embryogenesis. Importantly, Gsdf and Dmy were found to be co-localized in the same somatic cells in the XY gonads. When the XY embryos were treated with estradiol-17 beta, in order to reverse their phenotypic sex, a decline was observed in the expression of Gsdf in these embryos by real-time PCR. (C) 2010 Elsevier B.V. All rights reserved.