Control of the buoyancy of Microcystis aeruginosa via colony formation induced by regulating extracellular polysaccharides and cationic ions
Control of the buoyancy of Microcystis aeruginosa via colony formation induced by regulating extracellular polysaccharides and cationic ions
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DOI：
10.1007/s42452-019-1637-3
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发表时间：
2019-11
影响因子：
2.6
通讯作者：
Kai Wei;Sanghyeob Jung;Y. Amano;M. Machida
中科院分区：
文献类型：
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作者：
Kai Wei;Sanghyeob Jung;Y. Amano;M. Machida
气体囊泡影响微囊藻的浮力，控制微囊藻在水柱中的迁移。有些微囊菌没有囊泡，因此这些菌株不能从囊泡中获得浮力。在这项研究中，我们使用了两种类型的微囊菌，如非气体囊泡合成菌株m。aeruginosaUTEX-2061与气囊合成菌株m。铜绿脓毒杆菌-843在m浮力控制实验中的应用。绿脓杆菌。通过诱导菌落形成和计算各菌株的相对浮力率，证明了菌落形成与两种微囊菌株的浮力密切相关。含气囊泡的铜绿osies -843在较低钙、镁浓度下仍能获得浮力。不含气泡的aeruginosaUTEX-2061。在培养基中加入从微囊藻中提取的胞外多糖（EPS），M。aeruginosies -843和UTEX-2061在最低钙和镁浓度下可以表现出更高的浮力。结果表明，EPS与钙、镁等二价金属阳离子结合可有效地形成微囊菌落。钙、镁离子增强了微囊藻的集落形成能力，对浮力有显著影响。
It is well recognized that gas vesicles influence the buoyancy ofMicrocystisand control the migration in the water column. Some ofMicrocystisstrains do not have gas vesicles, so these strains cannot obtain buoyancy from them. In this study, we used two types ofMicrocystisstrains such as the non-gas vesicle synthesizing strainM. aeruginosaUTEX-2061 and the gas vesicle synthesizing strainM. aeruginosaNIES-843 in the buoyancy control experiments ofM. aeruginosa. By inducing colony formation and calculating the relative buoyancy rate of each strain, it was proven that the colony formation was strictly related to the buoyancy of bothMicrocystisstrains.M. aeruginosaNIES-843 possessing gas vesicles could obtain buoyancy under the lower concentration of calcium and magnesium thanM. aeruginosaUTEX-2061 possessing no gas vesicles. With the addition of the extracellular polysaccharides (EPS) extracted fromMicrocystisblooms into the culture medium,M. aeruginosaNIES-843 and UTEX-2061 could present higher buoyancy at the lowest concentrations of calcium and magnesium. It was suggested that EPS combined with the divalent metal cations such as calcium and magnesium madeMicrocystisform colony effectively. Calcium and magnesium ions enhanced the ability of colony formation ofMicrocystisand had a significant influence on buoyancy.