Helicobacter pylori modulation of gastric and duodenal mucosal T cell cytokine secretions in children compared with adults
Helicobacter pylori modulation of gastric and duodenal mucosal T cell cytokine secretions in children compared with adults
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DOI：
10.1046/j.1523-5378.2003.00147.x
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发表时间：
2003-06-01
期刊：
影响因子：
4.4
通讯作者：
Mascart, F
中科院分区：
文献类型：
--
作者：
Bontems, P;Robert, F;Mascart, F
背景资料。与成人相比，幽门螺杆菌感染的溃疡并不常见。孩子们。由于免疫决定因素影响幽门螺杆菌感染的结局，我们调查了幽门螺杆菌感染儿童的粘膜T细胞反应，并将其与成人和阴性对照进行比较。43例因消化不良症状接受上消化道内窥镜检查的患者进行了粘膜活检。流式细胞仪检测细胞因子释放浓度和CD3+、CD25+、CD69+细胞密度，ELISPOT法检测分泌细胞因子的细胞数。仅感染组分离的胃窦CD3+淋巴细胞数显著高于非感染组(P
Background. In contrast to adults, ulcers are uncommon in Helicobacter pylori-infected. children. Since immunological determinants influence the outcome of H. pylori infection, we have investigated mucosal T cell responses in H. pylori-infected children and compared them with those of adults and negative controls.Material and Methods. Mucosal biopsies were obtained from 43 patients undergoing an upper GI endoscopy for dyspeptic symptoms. The concentrations of released cytokines and the density of CD3+, CD25+ and CD69+ cells were evaluated by flow cytometry, and the numbers of cytokine-secreting cells were measured by ELISPOT.Results. The numbers of isolated antral CD3+ lymphocytes were only significantly raised in infected adults compared with noninfected controls (p