Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus
Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus
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DOI：
10.1128/jvi.73.5.3582-3586.1999
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发表时间：
1999-05-01
影响因子：
5.4
通讯作者：
Hino, S
中科院分区：
文献类型：
--
作者：
Miyata, H;Tsunoda, H;Hino, S
序列数据（H. Okamoto等，Hepatol，Res，10：1-16，1998），表明它不具有细小病毒典型的末端结构。阐明完整的基因组结构是必要的，以了解TTV的性质。我们获得了一个1.0 kb的扩增产物从血清样品的四个TTV携带者通过反向，巢式长PCR靶向核苷酸（nt）TTV的3025至3739和1至216。将从血清样品TA 278中获得的克隆的序列与GenBank中登记的序列进行比较。TTV基因组全长113 nt，为环状结构（nt 3740至3852 [=0]），形成一个117-nt的富含GC的片段（GC含量，在nt 3736至3852处为90.6%），我们发现36-nt的延伸（nt 3816至3851）与脊椎动物圆环病毒鸡贫血病毒（CAV）（M55918的nt 2237至2272）具有80.6%的相似性。推测的SP-1位点位于nt 3834 - 3839，随后是nt 85 - 90的TATA bos，nt 107 - 109的推测的VP 2的第一起始密码子，nt 2899 - 2901的推测的VP 1的终止密码子，和nt 3073 - 3078的poly（A）信号。这种安排与CAV的安排相似。TTV和CAV的GC富集区周围均存在AP-2、ATF/CREB结合位点和NF-κ B结合位点，提示TTV是第一种人类圆环病毒。
The sequence data (H. Okamoto et al,, Hepatol, Res, 10:1-16, 1998) of a neu ly discovered single-stranded DNA virus, TT virus ((TTV), showed that it did not have the terminal structure typical of a parvovirus. Elucidation of the complete genome structure was necessary to understand the nature of TTV. We obtained a 1.0-kb amplified product from serum samples of four TTV carriers by an inverted, nested long PCR targeted for nucleotides (nt) 3025 to 3739 and 1 to 216 of TTV. The sequence of a clone obtained from serum sample TA278 was compared with those registered in GenBank. The complete circular TTV genome contained a novel sequence of 113 nt (nt 3740 to 3852 [=0]) in between the known 3'- and 5'-end arms, forming a 117-nt GC-rich stretch (GC content, 90.6% at nt 3736 to 3852), We found a 36-nt stretch (nt 3816 to 3851) with an 80.6% similarity to chicken anemia virus (CAV) (nt 2237 to 2272 of M55918), a vertebrate circovirus. A putative SP-I site was located at nt 3834 to 3839, followed by a TATA bos at nt 85 to 90, the first initiation codon of a putative VP2 at nt 107 to 109, the termination codon of a putative VP1 at nt 2899 to 2901, and a poly(A) signal at nt 3073 to 3078. The arrangement was similar to that of CAV. Furthermore, several AP-2 and ATF/CREB binding sites and an NF-KB site were arranged around the GC-rich region in both TTV and CAV, The data suggested that TTV is circular and similar to CAV in its genomic organization, implying that TTV Is the first human circovirus.