Genome-wide screening of genes required for swarming motility in Escherichia coli K-12
Genome-wide screening of genes required for swarming motility in Escherichia coli K-12
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DOI：
10.1128/jb.01294-06
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发表时间：
2007-02-01
影响因子：
3.2
通讯作者：
Fukui, Kazuhiro
中科院分区：
文献类型：
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作者：
Inoue, Tetsuyoshi;Shingaki, Ryuji;Fukui, Kazuhiro
大肠埃希菌K-12具有在半固体介质上通过集群运动迁移的能力。系统而全面地收集了基因中断的大肠杆菌K-12突变体(Keio突变株)，以鉴定参与该细菌群体运动的基因。在3985个非必需基因突变体中，294个被发现表现出强烈的抑制-聚集表型。此外，在294个突变体中，有216个在游泳运动方面没有明显的缺陷；因此，这216个基因被认为是与集群表型特异相关的。与蜂群相关的基因被归类为不同的功能类别，表明蜂群是一种特殊的运动形式，需要广泛的细胞活动。这些基因包括三羧酸循环和葡萄糖代谢、铁获取、伴侣和蛋白质折叠催化剂、信号转导和细胞表面成分的生物合成基因，如内毒素、肠道细菌共同抗原和I型菌毛。脂多糖和肠道细菌共同抗原可能是重要的表面作用成分，有助于降低表面张力，从而促进大肠杆菌K-12菌株的群体迁移。
Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type I fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.