Isolation and Characterization of α-Glucosidase Inhibitor from the Fungus Ganoderma lucidum
Isolation and Characterization of α-Glucosidase Inhibitor from the Fungus Ganoderma lucidum
复制标题
真菌灵芝中α-葡萄糖苷酶抑制剂的分离和表征
作者：
Shin;Hong Joon Nho
从灵芝子实体中分离得到一种α -葡萄糖苷酶抑制剂SKG-3，并对其理化性质进行了表征。它是一种高度特异性和有效的可逆α -葡萄糖苷酶抑制剂。对α -葡萄糖苷酶有很强的抑制作用，IC50值为4.6微g/ml，对其他糖苷酶无抑制作用。透析后酶活性可以恢复，从而为抑制的可逆性提供了证据。Lineweaver-Burk图显示，SKG-3对α -葡萄糖苷酶的抑制是竞争性的。
An alpha-glucosidase inhibitor, SKG-3, was isolated from the fruiting bodies of Ganoderma lucidum and its physico-chemical properties were characterized. It was a highly specific and effective reversible inhibitor of alpha-glucosidase. It showed very potent inhibitory activity against alpha-glucosidase with an IC50 value of 4.6 micro g/ml, but no activity for any other glycosidases tested. Enzyme activity could be recovered upon dialysis, thus providing evidence for the reversibility of the inhibition. A Lineweaver-Burk plot indicated that the SKG-3 inhibition of alpha-glucosidase was competitive.