Real-time metabolic imaging
Real-time metabolic imaging
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DOI：
10.1073/pnas.0601319103
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发表时间：
2006-07-25
影响因子：
11.1
通讯作者：
Thaning, Mikkel
中科院分区：
文献类型：
--
作者：
Golman, Klaes;in't Zandt, Rene;Thaning, Mikkel
内源性物质丙酮酸是维持细胞内能量平衡的重要物质，为细胞生存所必需的几个重要代谢过程提供了窗口。因此，细胞活力反映在丙酮酸的新陈代谢中。到目前为止，核磁共振光谱学一直是了解丙酮酸在体内命运的唯一非侵入性方法，但即使在高场强下，核磁共振的灵敏度也很低，只能提供关于稳态条件的信息。关于注射后第一分钟内物质的分布、定位和代谢率的医学相关信息尚未获得。超极化技术的使用已经使C-13(1)在高达0.3M的丙酮酸盐溶液中极化了10%-15%。静脉注射。将该溶液注射到大鼠和猪体内，可以在大约10 S的时间内成像丙酮酸的分布及其主要代谢物乳酸和丙氨酸的图谱。核磁共振实时分子成像已成为现实。
The endogenous substance pyruvate is of major importance to maintain energy homeostasis in the cells and provides a window to several important metabolic processes essential to cell survival. Cell viability is therefore reflected in the metabolism of pyruvate. NMR spectroscopy has until now been the only noninvasive method to gain insight into the fate of pyruvate in the body, but the low NMR sensitivity even at high field strength has only allowed information about steady-state conditions. The medically relevant information about the distribution, localization, and metabolic rate of the substance during the first minute after the injection has not been obtainable. Use of a hyperpolarization technique has enabled 10-15% polarization of C-13(1) in up to a 0.3 M pyruvate solution. i.v. injection of the solution into rats and pigs allows imaging of the distribution of pyruvate and mapping of its major metabolites lactate and alanine within a time frame of approximate to 10 s. Real-time molecular imaging with MRI has become a reality.