Down-Regulation of SSSII-2 Gene Expression Results in Novel Low-Amylose Rice with Soft, Transparent Grains
Down-Regulation of SSSII-2 Gene Expression Results in Novel Low-Amylose Rice with Soft, Transparent Grains
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SSSII-2 基因表达下调导致籽粒柔软、透明的新型低直链淀粉水稻
DOI：
10.1021/acs.jafc.8b02913
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发表时间：
2018-09-19
影响因子：
6.1
通讯作者：
Liu, Qiao-Quan
中科院分区：
文献类型：
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作者：
Li, Qan-Feng;Huang, Li-Chun;Liu, Qiao-Quan
虽然软米具有低直链淀粉含量（AC），但其食用和烹饪品质（ECQ）较高，但由于胚乳不透明，其外观较差。在这里，一个新的软米低AC，但透明的外观产生敲低SSSII-2，一个基因编码的可溶性淀粉合成酶（SSS）的一种亚型的表达。SSSII-2 RNAi水稻的理化性质与对照有较大差异，但更接近于流行的软米“南京46”。食味值测定进一步证明，SSSII-2 RNAi水稻的ECQ与“南京46”相当，但只有SSSII-2 RNAi水稻在低水分条件下仍保持胚乳透明。进一步的研究表明，SSSII-2 RNAi水稻不同的淀粉颗粒形态和精细结构可能与其特性有关。因此，SSSII-2在未来的优质稻育种中具有潜在的应用价值。
Although soft rice, with low amylose content (AC), has high eating and cooking quality (ECQ), its appearance is poor due to the opaque endosperm. Here, a novel soft rice with low AC but a transparent appearance was generated by knocking-down the expression of SSSII-2, a gene encoding one isoform of soluble starch synthase (SSS). The physicochemical properties of the SSSII-2 RNAi rice are quite different from the control but more like the popular soft rice "Nanjing 46". The taste value assay further demonstrated that the ECQof SSSII-2 RNAi rice was as high as "Nanjing 46", but only SSSII-2 RNAi rice retained the transparent endosperm under low moisture conditions. Further examination showed that the different morphologies and fine structures of the starch granules may contribute to the specific properties of SSSII-2 RNAi rice. Therefore, SSSII-2 has potential application in future high quality rice breeding programs.