TELOMERIC DNA OLIGONUCLEOTIDES FORM NOVEL INTRAMOLECULAR STRUCTURES CONTAINING GUANINE GUANINE BASE-PAIRS
TELOMERIC DNA OLIGONUCLEOTIDES FORM NOVEL INTRAMOLECULAR STRUCTURES CONTAINING GUANINE GUANINE BASE-PAIRS
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DOI：
10.1016/0092-8674(87)90577-0
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发表时间：
1987-12-24
期刊：
影响因子：
64.5
通讯作者：
BLACKBURN, EH
中科院分区：
文献类型：
--
作者：
HENDERSON, E;HARDIN, CC;BLACKBURN, EH
分析了几种生物端粒单链分子末端的DNA寡核苷酸的结构特征。基于包括非变性聚丙烯酰胺凝胶电泳、吸光度热变性分析、1H和31P核磁共振波谱在内的物理研究，我们得出结论，这些分子可以通过形成非Watson-Crick、Ganine.cntdot.guanine配对的分子内结构而自缔合。这些结构在40度以下形成。C，在中等离子强度和中性pH下，在非变性聚丙烯酰胺凝胶中表现为发夹状双链。对来自四膜虫(T2G4)4的端粒序列形成的发夹结构的详细分析表明，它是一种独特的由氢键稳定的结构，在syn构象中含有G残基。我们认为这种新形式的DNA对端粒功能很重要，并为非Watson-Crick碱基配对DNA结构的生物学相关性开创了先例。
Structural properties of DNA oligonucleotides corresponding to the single-stranded molecular terminus of telomeres from several organisms were analyzed. Based on physical studies including nondenaturing polyacrylamide gel electrophoresis, absorbance thermal denaturation analysis, and 1H and 31P nuclear magnetic resonance spectroscopy, we conclude that these molecules can self-associate by forming non-Watson-Crick, guanine.cntdot.guanine based-paired, intramolecular structures. These structures form below 40.degree. C at moderate ionic strength and neutral pH and behave like hairpin duplexes in nondenaturing polyacrylamide gels. Detailed analysis of the hairpin structure formed by the telomeric sequence from Tetrahymena, (T2G4)4, shows that it is a unique structure stabilized by hydrogen bonds and contains G residues in the syn conformation. We propose that this novel form of DNA is important for telomere function and sets a precedent for the biological relevance of non-Watson-Crick base-paired DNA structures.