Relation of hypoxia-inducible factor-1α to vascular endothelial growth factor and vasoactive factors during healing of gastric ulcers
Relation of hypoxia-inducible factor-1α to vascular endothelial growth factor and vasoactive factors during healing of gastric ulcers
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DOI：
10.1097/01.fjc.0000166305.79055.ad
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发表时间：
2004-11-01
影响因子：
3
通讯作者：
Yamashito, K
中科院分区：
文献类型：
--
作者：
Hashimoto, H;Akimoto, M;Yamashito, K
探讨胃溃疡愈合过程中缺氧诱导因子-1α(HIF-1α)与血管内皮生长因子和血管活性因子的关系。胃溃疡分为活动期、愈合期和瘢痕期。HIF-1α、ET-1、诱导型一氧化氮合酶和血管内皮生长因子mRNA在溃疡愈合活动期的表达最高，内皮素-1、血管内皮生长因子蛋白水平和一氧化氮的表达在愈合期较高。因此，在胃溃疡愈合的活动期，HIF-1α的mRNA水平有升高的趋势，提示该因子参与了内皮素-1、诱导型一氧化氮合酶和血管内皮生长因子的诱导。此外，在溃疡愈合的不同阶段，HIF-1α的mRNA水平没有显著差异，在任何阶段都没有发现可检测到的HIF-1α蛋白水平。提示这些血管生成因子和血管活性物质可能是由HIF-1α诱导的。在胃镜检查的活跃期，考虑到溃疡愈合的初始阶段，溃疡愈合的过程已经开始，溃疡边缘的组织已经复氧。
We studied the relation of hypoxia-inducible factor-1alpha (HIF-1alpha) to vascular endothelial growth factor and vasoactive factors during the healing of gastric ulcers. The gastric ulcers were divided into three stages (active stage, healing stage and scar stage). The expression of HIF-1alpha, endothelin-1, inducible nitric oxide synthase, and vascular endothelial growth factor mRNA was highest during the active stage of ulcer healing, and endothelin-1, vascular endothelial growth factor protein levels and nitric oxide were higher during the healing stage. Thus, levels of HIF-1alpha mRNA tend to increase during the active stage of gastric ulcer healing, suggesting that this factor participates in the induction of endothelin-1, inducible nitric oxide synthase, and vascular endothelial growth factor. Also, the HIF-1alpha mRNA level did not differ significantly among the various stages of ulcer healing, and detectable levels of HIF-1alpha protein were not found during any stage. This suggests that these angiogenic factors and vasoactive substances may be induced by HIF-1alpha. During the active stage on endoscopic examination, considered the initial phase of ulcer healing, the process of ulcer healing has begun, and the tissue at the ulcer margin has already been reoxygenated.