Co-option of a default secretory pathway for plant immune responses
Co-option of a default secretory pathway for plant immune responses
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DOI：
10.1038/nature06545
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发表时间：
2008-02-14
期刊：
影响因子：
64.8
通讯作者：
Schulze-Lefert, Paul
中科院分区：
文献类型：
--
作者：
Kwon, Chian;Neu, Christina;Schulze-Lefert, Paul
细胞自主免疫广泛存在于植物-真菌相互作用中，并在细胞表面或病原体进入后终止真菌发病机制。虽然入侵后的抵抗反应通常与遭受寄生虫攻击的植物细胞的自给自足的细胞死亡同时发生，但这些细胞在入侵前的防御中幸存下来。拟南芥突变分析确定 PEN1 突触蛋白是针对子囊菌白粉病真菌的两种侵入前抗性途径的组成部分 (1-3)。在这里，我们发现质膜驻留的 PEN1 与 SNAP33 接头和囊泡相关膜蛋白 (VAMP) 子集一起形成 SDS 抗性可溶性 N-乙基马来酰亚胺敏感因子附着蛋白受体 (SNARE) 复合物。 PEN1依赖性抗病性在体内主要通过两个功能冗余的VAMP72亚家族成员VAMP721和VAMP722发挥作用。出乎意料的是，相同的两个 VAMP 蛋白也在默认分泌途径中冗余地起作用，这表明由于植物免疫默认途径的进化共同选择，它们在不同的生物过程中具有双重功能。分泌性 PEN1 - SNAP33 - VAMP721/722 复合物的抗病功能及其组分的病原体诱导的亚细胞动力学在机制上让人想起脊椎动物中的免疫突触形成，从而能够通过局灶分泌来执行免疫反应。
Cell- autonomous immunity is widespread in plant - fungus interactions and terminates fungal pathogenesis either at the cell surface or after pathogen entry. Although post- invasive resistance responses typically coincide with a self- contained cell death of plant cells undergoing attack by parasites, these cells survive pre- invasive defence. Mutational analysis in Arabidopsis identified PEN1 syntaxin as one component of two pre- invasive resistance pathways against ascomycete powdery mildew fungi(1-3). Here we show that plasma- membrane- resident PEN1 promiscuously forms SDS- resistant soluble N- ethylmaleimide sensitive factor attachment protein receptor ( SNARE) complexes together with the SNAP33 adaptor and a subset of vesicle- associated membrane proteins ( VAMPs). PEN1- dependent disease resistance acts in vivo mainly through two functionally redundant VAMP72 subfamily members, VAMP721 and VAMP722. Unexpectedly, the same two VAMP proteins also operate redundantly in a default secretory pathway, suggesting dual functions in separate biological processes owing to evolutionary co- option of the default pathway for plant immunity. The disease resistance function of the secretory PEN1 - SNAP33 - VAMP721/ 722 complex and the pathogen- induced subcellular dynamics of its components are mechanistically reminiscent of immunological synapse formation in vertebrates, enabling execution of immune responses through focal secretion.