Gender difference in levels of alpha2-adrenoceptor mRNA in the rat tail artery.
Gender difference in levels of alpha2-adrenoceptor mRNA in the rat tail artery.
复制标题
大鼠尾动脉中 α2-肾上腺素受体 mRNA 水平的性别差异。
DOI：
10.1016/s0014-2999(98)00948-0
复制
发表时间：
1999
影响因子：
5
通讯作者：
Duckles,SP
中科院分区：
文献类型：
--
作者：
McNeill,AM;Leslie,FM;Krause,DN;Duckles,SP
为了研究大鼠尾动脉收缩反应中不同mRNA水平是性别差异的基础这一假设，采用原位杂交法测定了α2肾上腺素受体mRNA。发现α2A和α 2C肾上腺素受体亚型的信使RNA定位于平滑肌层。未检测到α 2B肾上腺素受体亚型的mRNA。雌性尾动脉中α 2C-肾上腺素受体mRNA水平高于雄性尾动脉（417±35 vs. 263±38 dpm/mg，P=0.01），而两性中α 2A-肾上腺素受体mRNA水平相同。大鼠尾动脉中α2肾上腺素能受体mRNA水平可能与功能蛋白水平平行。
To investigate the hypothesis that differing mRNA levels underlie gender differences in the contractile response of the rat tail artery, α2-adrenoceptor mRNA was measured using in situ hybridization. Messenger RNA for the α2A- and α2C-adrenoceptor subtypes was found localized to the smooth muscle layer. There was no detectable mRNA present for the α2B-adrenoceptor subtype. Levels of α2C-adrenoceptor mRNA were greater in female compared to male tail arteries (417±35 vs. 263±38 dpm/mg, P=0.01), while levels of α2A-adrenoceptor mRNA were the same in both sexes. Levels of α2-adrenoceptor mRNA may parallel levels of functioning protein present in the rat tail artery.