Development of an In Vitro Assay for Detection of Drug-Induced Resuscitation-Promoting-Factor-Dependent Mycobacteria.
Development of an In Vitro Assay for Detection of Drug-Induced Resuscitation-Promoting-Factor-Dependent Mycobacteria.
复制标题
DOI：
10.1128/aac.00518-16
复制
发表时间：
2016-10
影响因子：
4.9
通讯作者：
Mukamolova GV
中科院分区：
文献类型：
--
作者：
Loraine J;Pu F;Turapov O;Mukamolova GV
结核病是一种主要的传染病，需要长期联合四种药物进行化疗。在这里，我们提出的数据表明，结核分枝杆菌是结核病的病原体，污垢分枝杆菌是一种广泛用于抗结核药物筛选的模式生物，用一线药物治疗可以产生大量的种群，这些种群只能通过添加生长中的分枝杆菌的培养上清液来恢复。这些细菌不能在标准培养基中生长，导致处理样本中活的分枝杆菌数量被严重低估。我们培育了高表达结核分枝杆菌复苏促进因子(RPFS)的耻垢分枝杆菌菌株，并展示了它们在检测药物暴露后产生的依赖于RPF的分枝杆菌中的应用。我们的数据为验证抗结核药物的杀菌活性提供了新的机会。
Tuberculosis is a major infectious disease that requires prolonged chemotherapy with a combination of four drugs. Here we present data suggesting that treatment of Mycobacterium tuberculosis, the causative agent of tuberculosis, and Mycobacterium smegmatis, a model organism widely used for the screening of antituberculosis agents, with first-line drugs resulted in the generation of substantial populations that could be recovered only by the addition of a culture supernatant from growing mycobacteria. These bacilli failed to grow in standard media, resulting in significant underestimation of the numbers of viable mycobacteria in treated samples. We generated M. smegmatis strains overexpressing M. tuberculosis resuscitation-promoting factors (Rpfs) and demonstrated their application for the detection of Rpf-dependent mycobacteria generated after drug exposure. Our data offer novel opportunities for validation of the sterilizing activity of antituberculosis agents.