Survey of major capsid genes (g23) of T4-type bacteriophages in rice fields in Northeast China
Survey of major capsid genes (g23) of T4-type bacteriophages in rice fields in Northeast China
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DOI：
10.1016/j.soilbio.2008.11.012
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发表时间：
2009-02
影响因子：
9.7
通讯作者：
Guanghua Wang;J. Jin;S. Asakawa;M. Kimura
中科院分区：
文献类型：
--
作者：
Guanghua Wang;J. Jin;S. Asakawa;M. Kimura
利用引物MZIA1bis和MZIA6对东北15个水稻土壤中提取的DNA中t4型噬菌体衣壳基因（g23）进行了分析。变性梯度凝胶电泳（DGGE）分离pcr扩增的g23产物。在g23个无性系中共鉴定出53个DGGE条带，其中9个属于东北特有的新类群。此外，4个和6个无性系与先前从日本稻田获得的无性系形成了两个新的类群。其余无性系大部分属于水稻类群I和V，水稻类群II、III、IV和VI均无克隆，说明g23基因在东北稻田的系统发育分布与日本稻田不同。
We surveyed the capsid genes (g23) of T4-type bacteriophages in DNA extracted from fifteen rice field soils in Northeast China using primers MZIA1bis and MZIA6. Denaturing gradient gel electrophoresis (DGGE) was performed to separate PCR-amplified g23 products. In total, 53 DGGE bands were identified as g23 clones, nine of which belonged to a novel, Northeast China-specific group. In addition, four and six clones formed two novel groups with previously ungrouped clones obtained from Japanese rice fields. The majority of the remaining clones fell into Paddy Groups I and V, none of the clones belonged to Paddy Groups II, III, IV, and VI, indicating that phylogenetic distribution of g23 genes in rice fields in Northeast China was different from that in Japanese rice fields.