Increased activity of the tandem fructose-1,6-bisphosphate aldolase, triosephosphate isomerase and fructose-1,6-bisphosphatase enzymes in Anabaena sp strain PCC 7120 stimulates photosynthetic yield
Increased activity of the tandem fructose-1,6-bisphosphate aldolase, triosephosphate isomerase and fructose-1,6-bisphosphatase enzymes in Anabaena sp strain PCC 7120 stimulates photosynthetic yield
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鱼腥藻串联果糖 1,6-二磷酸醛缩酶、磷酸丙糖异构酶和果糖 1,6-二磷酸酶的活性增加。
DOI：
10.1007/s10811-007-9286-0
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发表时间：
2008-08-01
影响因子：
3.3
通讯作者：
Chen, Haibao
中科院分区：
文献类型：
--
作者：
Ma, Weimin;Wei, Lanzhen;Chen, Haibao
在遗传水平上对光合产量的调控主要集中在通过基因工程对卡尔文循环中的催化酶的操纵上。为了研究卡尔文循环中酶活性增加对光合产量的贡献，将水稻果糖-1，6-二磷酸醛缩酶（FBA）、菠菜磷酸丙糖异构酶（TPI）和小麦果糖-1，6-二磷酸酶（FBPase）基因串联克隆，并在蓝藻Anabaena sp.菌株PCC 7120细胞中共过表达。FBA，TPI和FBPase的酶活性，以及景天庚酮糖-1，7-二磷酸酶（SBP 1），在转基因细胞中相对于野生型显着增加。光合产量，反映了光合O2的演变和干细胞的重量，也显着增加转基因细胞与野生型细胞。SBBP的活性被认为是卡尔文循环中1，5-二磷酸核酮糖（RuBP）再生的最重要因素，转基因细胞中TPI单独活性的增加不会刺激光合产量。因此，FBA和FBPase的活性增加，而不是TPI，通过刺激SBBP活性，从而加速RuBP再生速率，显着提高转基因细胞的光合产量。
The regulation of photosynthetic yield at the genetic level has largely focused on manipulation of the catalytic enzymes in the Calvin cycle by genetic engineering. In order to investigate the contribution of increased enzymatic activity in the Calvin cycle on photosynthetic yield, the rice fructose-1,6-bisphosphate aldolase (FBA), spinach triosephosphate isomerase (TPI) and wheat fructose-1,6-bisphosphatase (FBPase) genes were cloned in tandem and co-overexpressed in cyanobacterium Anabaena sp. strain PCC 7120 cells. The enzymatic activities of FBA, TPI and FBPase, as well as sedoheptulose-1,7-bisphosphatase (SBPase), were remarkably increased in transgenic cells relative to the wild-type. The photosynthetic yield, as reflected by photosynthetic O2 evolution and dry cellular weight, was also markedly increased in transgenic cells versus wide-type cells. The activity of SBPase is considered the most important factor for ribulose-1,5-bisphosphate (RuBP) regeneration in the Calvin cycle, and increased activity of TPI alone in transgenic cells does not stimulate photosynthetic yield. Thus, the increased activity of FBA and FBPase, but not TPI, significantly improved photosynthetic yield in transgenic cells by stimulating SBPase activity and consequently accelerating the RuBP regeneration rate.