IDENTIFICATION OF TWO PIWI GENES AND THEIR EXPRESSION PROFILE IN HONEYBEE, Apis mellifera
IDENTIFICATION OF TWO PIWI GENES AND THEIR EXPRESSION PROFILE IN HONEYBEE, Apis mellifera
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蜜蜂 (Apis mellifera) 中两个 piwi 基因的鉴定及其表达谱。
DOI：
10.1002/arch.20362
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发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Han, Zhaojun
中科院分区：
文献类型：
--
作者：
Liao, Zhen;Jia, Qidong;Han, Zhaojun
Piwi基因参与一类新的非编码RNA piRNA的生物合成，在精子发生和卵子发生中起着重要的调控作用。然而，这些基因在大多数昆虫中还没有得到很好的理解。为了了解piwi基因在蜜蜂繁殖中的功能，我们扩增了两个全长piwi样基因Am-aub和Am-ago 3。克隆的Am-aub和Am-ago 3基因均含有典型的PAZ和PIWI结构域和活性催化基序“Asp-Asp-Asp/His/Glu/Lys”，表明这两个piwi样基因具有切割酶活性。我们通过定量PCR检测了Am-aub和Am-ago 3在工蜂、蜂王、雄蜂和雌性幼虫中的表达水平。在所有测试样品中Am-aub比Am-ago 3更丰富。Am-aub和Am-ago 3在雄蜂中高度表达，但在工蜂和蚁后中不表达。重要的发现是幼虫食物流影响成年蜜蜂中Piwi基因的表达。这有助于在分子水平上理解蜜蜂生殖状态的营养控制。(C)2010 Wiley Periodicals，Inc.
Piwi genes play an important role in regulating spermatogenesis and oogenesis because they participate in the biogenesis of piRNAs, a new class of noncoding RNAs. However, these genes are not well understood in most insects. To understand the function of piwi genes in honeybee reproduction, we amplified two full-length piwi-like genes, Am-aub and Am-ago3. Both the cloned Am-aub and Am-ago3 genes contained typical PAZ and PIWI domains and active catalytic motifs "Asp-Asp-Asp/His/Glu/Lys,'' suggesting that the two piwi-like genes possessed slicer activity. We examined the expression levels of Am-aub and Am-ago3 in workers, queens, drones, and female larvae by quantitative PCR. Am-aub was more abundant than Am-ago3 in all the tested samples. Both Am-aub and Am-ago3 were highly expressed in drones but not in workers and queens. The significant finding was that the larval food stream influenced the expression of Piwi genes in adult honeybees. This helps to understand the nutritional control of reproductive status in honeybees at the molecular level. (C) 2010 Wiley Periodicals, Inc.