Gonadotropin-releasing hormone (GnRF), Molluscan cardioexcitatory peptide (FMRFamide), enkephalin and related neuropeptides affect goldfish retinal ganglion cell activity
Gonadotropin-releasing hormone (GnRF), Molluscan cardioexcitatory peptide (FMRFamide), enkephalin and related neuropeptides affect goldfish retinal ganglion cell activity
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促性腺激素释放激素（GnRF）、软体动物心脏兴奋肽（FMRFamide）、脑啡肽及相关神经肽影响金鱼视网膜神经节细胞活性
DOI：
10.1016/0006-8993(86)91162-5
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
W. Stell
中科院分区：
文献类型：
--
作者：
S. E. Walker;W. Stell
最近，研究人员利用免疫细胞化学方法将促性腺激素释放激素（GnRF）样化合物和软体动物心兴奋肽（FMRFamide）样化合物共定位于金鱼的末段神经，这是一种被认为是嗅觉-视网膜传出系统的物质。在目前的研究中，这些和相关的神经肽被证明影响神经节细胞的活动，记录在细胞外，当应用于分离的金鱼视网膜。GnRF通常是令人兴奋的。鲑鱼GnRF （sGnRF）的效力是鸡或哺乳动物GnRF的10 - 30倍。FMRFamide和脑啡肽也经常兴奋，但引起的反应比sGnRF更多样化。含有脑啡肽和FMRFamide序列的M Met5-enkephalin-Arg6-Phe7-NH2（YGGFMRFamide）倾向于像这两种肽一样起作用，但主要具有脑啡肽的性质。神经肽Y和胰多肽c端六肽（其c端（-Arg-Tyr-NH2）与FMRFamide的c端（- arg - ph - nh2）密切相关）没有一致的反应。阈值剂量相当于：sGnRF为0.1 μM；0.5 μM用于YGGFMRFamide；1.5 μM的FMRFamide和脑啡肽。单次、10 - 20倍阈值剂量的sGnRF诱导快速、完全、不可逆脱敏；但对其他肽的脱敏不常见且有限。总的来说，所有测试的肽对空间和颜色上拮抗的感受野成分的影响相似，但在FMRFamide和阿片拮抗剂纳洛酮的少数情况下，可以看到选择性作用。尤其是对sGnRF和FMRFamide的反应，往往在冬季和春季最常见和最明显，这表明与季节性调节的性和生殖活动有关。我们的观察结果进一步证明了与sGnRF和FMRFamide相关的神经肽在硬骨末梢神经中的递质样作用。激动剂和拮抗剂的作用，单独或联合，强烈暗示在金鱼视网膜中存在独特的突触后受体和/或GnRF-， FMRFamide-和脑啡肽样肽的神经通路。
Recently gonadotropin-releasing hormone (GnRF)-like and molluscan cardioexcitatory peptide (FMRFamide)-like compounds have been colocalized immunocytochemically to the terminal nerve, a presumed olfactoretinal efferent system in goldfish. In the present study these and related neuropeptides were shown to affect ganglion cell activity, recorded extracellularly, when applied to the isolated superfused goldfish retina. GnRF was usually excitatory. Salmon GnRF (sGnRF) was 10–30× more potent than chicken or mammalian GnRF. FMRFamide and enkephalin also were often excitatory but caused more varied responses than sGnRF. M Met5-enkephalin-Arg6-Phe7-NH2(YGGFMRFamide), which contains both enkephalin and FMRFamide sequences, tended to act like both of these peptides but with mainly enkephalin-like properties. Neuropeptide Y and the C-terminal hexapeptide of pancreatic polypeptides, whose C-terminus (-Arg-Tyr-NH2) is closely related to that of FMRFamide (-Arg-Phe-NH2), gave no consistent responses. Threshold doses were equivalent to: 0.1 μM for sGnRF; 0.5 μM for YGGFMRFamide; 1.5 μM for FMRFamide and enkephalin. Rapid, complete and irreversible desensitization was induced by single, 10–20× threshold doses of sGnRF; but desensitization was infrequent and limited with the other peptides. In general, all peptides tested affected the spatially and chromatically antagonistic receptive field components similarly, but selective actions were seen in a few cases with FMRFamide and with the opioid antagonist, naloxone. Responses, especially to sGnRF and FMRFamide, tended to be most frequently obtained and pronounced in winter and spring, suggesting a correlation with seasonally regulated sexual and reproductive activity. Our observations provide further evidence for transmitter-like roles of neuropeptides related to sGnRF and FMRFamide in the teleostean terminal nerve. The actions of agonists and antagonists, singly and in combination, imply strongly that there are distinctive postsynaptic receptors and/or neural pathways for GnRF-, FMRFamide- and enkephalin-like peptides in the goldfish retina.
神经元功能中的肽：使用青蛙自主神经节的研究。
DOI：
10.1101/sqb.1983.048.01.040
发表时间：
1983
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Jan,YN;Bowers,CW;Branton,D;Evans,L;Jan,LY
通讯作者：
Jan,LY
爬行动物胰岛素、胰高血糖素和胰多肽的分离和表征：鳄鱼（密西西比鳄）胰岛素和胰多肽的完整氨基酸序列。
影响因子：
2.7
作者：
Lance,V;Hamilton,JW;Rouse,JB;Kimmel,JR;Pollock,HG
通讯作者：
Pollock,HG
硬骨鱼大脑中存在三种类似黄体生成素释放激素的物质：与哺乳动物的 LH-RH 十肽均不相同。
DOI：
10.1016/s0006-291x(82)80032-6
发表时间：
1982
影响因子：
3.1
作者：
Barnett,FH;Sohn,J;Reichlin,S;Jackson,IM
通讯作者：
Jackson,IM