LIPID STRUCTURAL ORDER PARAMETERS (RECIPROCAL OF FLUIDITY) IN BIOMEMBRANES DERIVED FROM STEADY-STATE FLUORESCENCE POLARIZATION MEASUREMENTS
LIPID STRUCTURAL ORDER PARAMETERS (RECIPROCAL OF FLUIDITY) IN BIOMEMBRANES DERIVED FROM STEADY-STATE FLUORESCENCE POLARIZATION MEASUREMENTS
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DOI：
10.1016/0005-2736(81)90390-4
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
VANDERMEER, BW
中科院分区：
文献类型：
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作者：
VANBLITTERSWIJK, WJ;VANHOEVEN, RP;VANDERMEER, BW
本文提出了一种解释的荧光偏振测量的脂质膜标记的非极性探针1，6-二苯基-1，3，5-己三烯。稳态荧光各向异性r S分解为快速衰减或动力学分量r f和无限慢衰减或静态分量r∞。后一种贡献在生物膜中占主导地位，完全由膜非极性区域的分子堆积程度（有序）决定; r∞与脂质有序参数的平方成比例。给出了rS与r∞之间的经验关系，这与基于液晶转动动力学理论的预测相一致。这种关系使我们能够直接从简单的稳态荧光偏振测量各种孤立的生物膜来估计脂质结构的有序参数。结果表明，决定生物膜有序参数的主要因素是温度，胆固醇和鞘磷脂含量和（在少数系统中）膜固有蛋白。
This paper presents an interpretation of fluorescence polarization measurements in lipid membranes which are labelled with the apolar probe 1, 6-diphenyl-1, 3, 5-hexatriene. The steady-state fluorescence anisotropy, r S, is resolved into a fast decaying or kinetic component, r f, and an infinitely slow decaying or static component, r∞. The latter contribution, which predominates in biological membranes, is exclusively determined by the degree of molecular packing (order) in the apolar regions of the membrane; r∞ is proportional to the square of the lipid order parameter. An empirical relation between r S and r∞ is presented, which is in agreement with a prediction based on a theory of rotational dynamics in liquid crystals. This relation enabled us to estimate a lipid structural order parameter directly from simple steady-state fluorescence polarization measurements in a variety of isolated biological membranes. It is shown that major factors determining the order parameter in biomembranes are the temperature, the cholesterol and sphingomyelin content and (in a few systems) the membrane intrinsic proteins.